An analytical method for the determination of perfluorinated compounds in whole blood using acetonitrile and solid phase extraction methods

• Published in: Journal of Chromatography A Vol. 1216; no. 25; pp. 4950 - 4956
• Main Authors: Yeung, Leo W.Y., Taniyasu, Sachi, Kannan, Kurunthachalam, Xu, Della Z.Y., Guruge, Keerthi S., Lam, Paul K.S., Yamashita, Nobuyoshi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.06.2009; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Acetonitrile; Acetonitriles - chemistry; Alkanesulfonic Acids - blood; Analytical chemistry; Animals; Biological and medical sciences; Chemical and industrial products toxicology. Toxic occupational diseases; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Exact sciences and technology; Fluorocarbons - blood; Formates - chemistry; Formic acid; Humans; Medical sciences; Other chromatographic methods; Perfluorooctanesulfonate; Reproducibility of Results; Solid phase extraction; Solid Phase Extraction - methods; Sulfonamides - blood; Taurodeoxycholic acid; Taurodeoxycholic Acid - blood; Toxicology; Various organic compounds; Whole blood; Whole blood; Solid phase extraction; Formic acid; Acetonitrile; Perfluorooctanesulfonate; Taurodeoxycholic acid; Steroid; Biological fluid; Chemical analysis; HPLC chromatography; Solvent extraction; Electrospray; Ion exchange chromatography; Chemical enrichment; Sample preparation; Bile acid; Quantitative analysis; Trace analysis; Organic perhalocompound; Coupled method; Interference; Anionic surfactant; Reversed phase chromatography; Animal; Fluorine Organic compounds; Conjugated compound; Mass spectrometry
• ISSN: 0021-9673; 1873-3778; 1873-3778
• DOI: 10.1016/j.chroma.2009.04.070

A method for the analysis of perfluorinated compounds (perfluoroalkyl sulfonates: C4, C6, C8, C10; perfluoroalkyl sulfinates: C6, C8, C10; perfluorooctanesulfonamide, N-ethyl perfluorooctanesulfonamide, N-ethyl perfluorooctanesulfonamidoacetate, perfluorocarboxylates: C4–C14; fluorotelomer carboxylate (7:3, 8:2) in whole blood using acetonitrile and OASIS WAX ® solid phase extraction (SPE) cartridge was developed. Separation of target compounds in two HPLC columns (ion exchange JJ50-2D and C18 Betasil columns) was examined. Matrix recoveries of the developed methods ranged from 70% to 120%. Separation of possible inferences such as taurodeoxycholic acid (TDC) was accomplished using an ion exchange JJ50-2D column, and this separation was validated using whole blood of different animals.