Osteoclasts are involved in the maintenance of dormant leukemic cells

• Published in: Leukemia research Vol. 34; no. 6; pp. 793 - 799
• Main Authors: Yokota, Asumi, Kimura, Shinya, Tanaka, Ruriko, Takeuchi, Miki, Yao, Hisayuki, Sakai, Kazuki, Nagao, Rina, Kuroda, Junya, Kamitsuji, Yuri, Kawata, Eri, Ashihara, Eishi, Maekawa, Taira
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2010
• Subjects: Abl protein; Animals; Animals, Newborn; BCR protein; Bone and Bones - physiology; Bone marrow; Bone marrow microenvironment; Bone matrix; Cell Cycle - drug effects; Cell Cycle - genetics; Cell Cycle - physiology; Cell proliferation; Cell Proliferation - drug effects; Cells, Cultured; Coculture Techniques; Fusion protein; Fusion Proteins, bcr-abl - genetics; Fusion Proteins, bcr-abl - metabolism; Hematology, Oncology and Palliative Medicine; Hemopoiesis; Humans; Ki-67 Antigen - metabolism; Leukemia; Leukemia - genetics; Leukemia - metabolism; Leukemia - pathology; Leukemia - physiopathology; Mice; Mice, Inbred BALB C; Microenvironments; Osteoblasts; Osteoblasts - metabolism; Osteoblasts - physiology; Osteoclasts; Osteoclasts - metabolism; Osteoclasts - physiology; Stem Cell Niche - drug effects; Stem Cell Niche - pathology; Stem cells; TGF-β 1; Transforming Growth Factor beta1 - pharmacology; Transforming growth factor- beta; Osteoclasts; TGF-β 1; Osteoblasts; Bone marrow microenvironment
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/j.leukres.2009.08.034

Abstract Osteoclasts (OCs) are specialized cells for the resorption of bone matrix that have also been recently reported to be involved in the mobilization of hematopoietic progenitor cells. When Ba/F3 cells expressing wild-type bcr-abl were co-cultured with osteoblasts (OBs), OCs, and bone slices, their proliferation was significantly suppressed, and the Ki-67 negative population, which is believed to be in G0 phase, was increased. The results of our in vitro experiments suggest that OCs could be involved in the maintenance of dormant leukemic cells in the bone marrow (BM) microenvironment through the release of soluble factors, one of which could be TGF-β.