Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma

• Published in: Nature communications Vol. 12; no. 1; p. 6058
• Main Authors: Pu, Weilin, Shi, Xiao, Yu, Pengcheng, Zhang, Meiying, Liu, Zhiyan, Tan, Licheng, Han, Peizhen, Wang, Yu, Ji, Dongmei, Gan, Hualei, Wei, Wenjun, Lu, Zhongwu, Qu, Ning, Hu, Jiaqian, Hu, Xiaohua, Luo, Zaili, Li, Huajun, Ji, Qinghai, Wang, Jiucun, Zhang, Xiaoming, Wang, Yu-Long
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.10.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 38/39; 631/1647/514/1949; 631/67/1459/1843; 631/67/2329; 631/67/69; Angiogenesis; Antiangiogenics; Cancer; Crosstalk; Ecosystems; Fibroblasts; Gene sequencing; Humanities and Social Sciences; Immunotherapy; Iodine; Iodine radioisotopes; Lymph nodes; Mesenchyme; Metastases; Microenvironments; Morphology; multidisciplinary; Papillary thyroid carcinoma; Patients; Phenotypes; Science; Science (multidisciplinary); Thyrocytes; Thyroid; Thyroid cancer; Transcriptomes; Tumor microenvironment; Tumors
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-26343-3

The tumor ecosystem of papillary thyroid carcinoma (PTC) is poorly characterized. Using single-cell RNA sequencing, we profile transcriptomes of 158,577 cells from 11 patients’ paratumors, localized/advanced tumors, initially-treated/recurrent lymph nodes and radioactive iodine (RAI)-refractory distant metastases, covering comprehensive clinical courses of PTC. Our data identifies a “cancer-primed” premalignant thyrocyte population with normal morphology but altered transcriptomes. Along the developmental trajectory, we also discover three phenotypes of malignant thyrocytes (follicular-like, partial-epithelial-mesenchymal-transition-like, dedifferentiation-like), whose composition shapes bulk molecular subtypes, tumor characteristics and RAI responses. Furthermore, we uncover a distinct BRAF -like-B subtype with predominant dedifferentiation-like thyrocytes, enriched cancer-associated fibroblasts, worse prognosis and promising prospect of immunotherapy. Moreover, potential vascular-immune crosstalk in PTC provides theoretical basis for combined anti-angiogenic and immunotherapy. Together, our findings provide insight into the PTC ecosystem that suggests potential prognostic and therapeutic implications. The characterisation of the papillary thyroid carcinoma (PTC) tumour microenvironment remains crucial. Here, the authors perform single-cell RNA sequencing in 11 patients and identify potential opportunities for the use of immunotherapy and its combination with anti-angiogenic therapy in PTC.