Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene

• Published in: Scientific reports Vol. 11; no. 1; pp. 17087 - 8
• Main Authors: Trabasso, Plinio, Matsuzawa, Tetsuhiro, Arai, Teppei, Hagiwara, Daisuke, Mikami, Yuzuru, Moretti, Maria Luiza, Watanabe, Akira
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.08.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326/193/2541; 692/699/255/1672; 704/172/4081; Aspergillus fumigatus; Drug resistance; Fungicides; Gene amplification; GNP; Gross National Product; Humanities and Social Sciences; Mortality; multidisciplinary; Mutation; Nucleic acids; Pesticides; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-96651-7

Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets . It also differentiated strains with TR 46 tandem repeats from those with TR 34 tandem repeats. These results showed this TR 46 -LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to A. fumigatus with TR 46 tandem repeats.