Cartilage Regeneration of Adipose-Derived Stem Cells in a Hybrid Scaffold from Fibrin-Modified PLGA

Adipose-derived stem cells (ASCs) appear to be a useful stem cell population, which has been shown to possess multipotentiality. The aim of this study was to evaluate the utility of ASCs in tissue-engineered cartilage using a hybrid scaffold from fibrin-modified PLGA scaffold. ASCs were isolated fro...

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Published inCell transplantation Vol. 18; no. 2; pp. 159 - 170
Main Authors Wei, Yiyong, Hu, Hunyu, Wang, Haiqiang, Wu, Yinsong, Deng, Lianfu, Qi, Jin
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.02.2009
SAGE Publishing
Subjects
Adipose Tissue - cytology
Animals
Cartilage, Articular - cytology
Cartilage, Articular - physiology
Cell Differentiation
Cell Survival
Chondrocytes - cytology
Collagen Type II - metabolism
Fibrin
Lactic Acid
Polyglycolic Acid
Polylactic Acid-Polyglycolic Acid Copolymer
Proteoglycans - metabolism
Rabbits
Regeneration
Tissue Scaffolds
Cartilage regeneration
Fibrin
Adipose
PLGA
Scaffold
Stem cells
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Summary:Adipose-derived stem cells (ASCs) appear to be a useful stem cell population, which has been shown to possess multipotentiality. The aim of this study was to evaluate the utility of ASCs in tissue-engineered cartilage using a hybrid scaffold from fibrin-modified PLGA scaffold. ASCs were isolated from rabbit adipose tissue. The PLGA scaffold was prepared by low-temperature deposition technology and the hybrid scaffold was fabricated by a freeze-drying method. When ASCs were seeded onto fibrin-modified PLGA scaffold in vitro, enhanced cellular viability was observed compared to unmodified PLGA scaffold. The analysis of proteoglycan and collagen II revealed that fibrin-modified scaffold succeeded in inducing ASCs to differentiate into chondrocytes in vitro. A preliminary study on cartilage regeneration was also performed in vivo. Observation of histology and immunoblotting demonstrated that ASCs containing the hybrid scaffold promoted cartilage regeneration in the defects of articular cartilage much better than other groups. These results indicated that ASCs containing the hybrid scaffold are a more effective way to potentially enhance articular cartilage regeneration.
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ISSN:0963-6897
1555-3892
DOI:10.3727/096368909788341261