Fluorometric Determination of Pyridoxal and its 5′-Phosphate in Biological Materials

A method of fluorometric determination of Pyridoxal and pyridoxal phosphate in biological materials was described. Samples were deproteinized with trichloroacetic acid, and pyridoxal was separated and concentrated by Dowex 1×8 and Amberlite CG 120 column treatment, treated with KCN at pH 7.5 and the...

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Published inJournal of vitaminology Vol. 16; no. 2; pp. 132 - 136
Main Authors TAKANASHI, SHIGERU, MATSUNAGA, ISAO, TAMURA, ZENZO
Format Journal Article
LanguageEnglish
Published Japan THE VITAMIN SOCIETY OF JAPAN 01.01.1970
Subjects
Animals
Cattle
Fluorometry
Humans
Hydrogen-Ion Concentration
Kidney - analysis
Liver - analysis
Pyridoxal Phosphate - analysis
Pyridoxal Phosphate - blood
Pyridoxine - analysis
Pyridoxine - blood
Rabbits
Rats
Trichloroacetic Acid
Vitamins - pharmacology
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Summary:A method of fluorometric determination of Pyridoxal and pyridoxal phosphate in biological materials was described. Samples were deproteinized with trichloroacetic acid, and pyridoxal was separated and concentrated by Dowex 1×8 and Amberlite CG 120 column treatment, treated with KCN at pH 7.5 and the fluorescence intensity was measured at pH 10. Pyridoxal phosphate was determined as pyridoxal after hydrolysis by acid phosphatase.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-5398
DOI:10.5925/jnsv1954.16.132