Studies on phosphorus solubilizing activity of a strain of phosphobacteria isolated from chestnut type soil in China

• Published in: Bioresource technology Vol. 99; no. 14; pp. 6702 - 6707
• Main Authors: Chen, Zixi, Ma, Shengwu, Liu, Lily(Lili)
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.09.2008; [New York, NY]: Elsevier Ltd; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Bacteria - growth & development; Bacteria - metabolism; Biofertilizer; Biological and medical sciences; China; Fertilizers; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Microscopy, Electron, Scanning; Other nutrients. Amendments. Solid and liquid wastes. Sludges and slurries; Phosphobacteria; Phosphorus - metabolism; Phosphorus-solubilizing ability; Plant growth; Soil fertility; Soil Microbiology; Soil-plant relationships. Soil fertility. Fertilization. Amendments; Solubility; Spores, Bacterial; Triticum - growth & development; Triticum aestivum; Asia; China; Biofertilizer; Phosphobacteria; Plant growth; Soil fertility; Phosphorus-solubilizing ability; Growth; Chestnut soil; Phosphorus; Organic fertilizer; Solubilization; Biological fertilizer; Plant; Bacterial fertilizer; Bacteria
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2007.03.064

A phosphorus solubilizing bacterium, designated phosphobacterium 9320-SD, was isolated from field soil in Tianjin, China. Cells of the phosphobacterium 9320-SD were gram-positive, rod shaped, and produced spores. When 9320-SD was inoculated into MPMLM, amended with powdered (insoluble) mineral phosphate as the single P source, and incubated at 30 °C, the release of soluble phosphorus increased with increasing amounts of added phosphates over the range of 0.12–4% (w/v). The maximal available phosphorus reached 12.01 mmol P/L after 7 days incubation. Furthermore, there was a direct positive correlation ( r = 0.9330) between the level of soluble phosphorus release and the concentration of viable bacteria. SEM study of the phosphate powder retrieved from the phosphobacterium 9320-SD cultured medium revealed the actual dissolution of phosphate from the mineral surface. Phosphobacterium 9320-SD had significant effect ( p < 0.05) on winter wheat total P and plant biomass under both pot and field conditions, although no obvious difference in plant height was found compared to the control. Taken together, these results demonstrate that phosphobacterium 9320-SD has the ability to convert non-available forms of phosphorus into plant-available forms, and therefore holds great potential for development as a biofertilizer to enhance soil fertility and promote plant growth.