Contractile activity-induced oxidative stress: cellular origin and adaptive responses
Department of Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom Previous studies have reported that oxidizing free radical species are generated during exercise, and there has been considerable interest in the potential effects of these on exercising tissues. We hypothesized that...
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Published in | American Journal of Physiology: Cell Physiology Vol. 280; no. 3; pp. C621 - C627 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.03.2001
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Subjects | |
Online Access | Get full text |
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Summary: | Department of Medicine, University of Liverpool, Liverpool L69 3GA,
United Kingdom
Previous studies have reported that
oxidizing free radical species are generated during exercise, and there
has been considerable interest in the potential effects of these on
exercising tissues. We hypothesized that contracting skeletal muscle
was a major source of oxidizing free radical species and that untrained
skeletal muscle would adapt to the oxidative stress of a single short
period of contractile activity by upregulation of the activity of
cytoprotective proteins in the absence of overt cellular damage.
Fifteen minutes of aerobic contractile activity was found to induce a
rapid release of superoxide anions from mouse skeletal muscle in vivo,
and studies with contracting cultured skeletal muscle myotubes
confirmed that this was due to release from myocytes rather than other
cell types present within muscle tissue in vivo. This increased oxidant
production caused a rapid, transient reduction in muscle protein thiol
content, followed by increases in the activities of superoxide
dismutase and catalase and in content of heat shock proteins. These
changes occurred in the absence of overt damage to the muscle cells.
superoxide; redox regulation; stress proteins |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0363-6143 1522-1563 |
DOI: | 10.1152/ajpcell.2001.280.3.c621 |