Identification of differentially expressed microRNAs as potential biomarkers for carcinoma ex pleomorphic adenoma

Carcinoma ex pleomorphic adenoma (CXPA) is a rare malignancy that transforms from PA. Early detection of the carcinoma by biopsy is difficult due to similar histopathology of the malignant and benign components. To address this, we investigated and compared the characteristic miRNA expression patter...

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Published inScientific reports Vol. 12; no. 1; p. 13383
Main Authors Kim, Hyojin, Eun, Shin, Jeong, Woo-Jin, Ahn, Soon-Hyun, Bae, Yun Jung, Lee, Joong Seob, Kim, Heejin
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 04.08.2022
Nature Publishing Group
Nature Portfolio
Subjects
631/337
631/67
692/4028
692/420
692/53
Adenoma
Biomarkers
Biopsy
Cancer
Histopathology
Humanities and Social Sciences
Malignancy
MicroRNAs
miRNA
multidisciplinary
Science
Science (multidisciplinary)
Tumor suppressor genes
Tumors
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Summary:Carcinoma ex pleomorphic adenoma (CXPA) is a rare malignancy that transforms from PA. Early detection of the carcinoma by biopsy is difficult due to similar histopathology of the malignant and benign components. To address this, we investigated and compared the characteristic miRNA expression patterns across samples of the PA, carcinomatous portions (CA) of CXPA, as well as conventional PA. We selected 13 CXPA and 16 conventional PA FFPE samples, separated the PA and CA portions of CXPA samples and conducted miRNA profiling for each group. Among 13 transcripts that were differentially expressed between PA and CA of CXPA, eight miRNAs were up-regulated and five down-regulated in CA. Bioinformatic analysis revealed that the up-regulated miRNAs were related to cancer progression and down-regulated ones to tumor suppression. Additionally, seven miRNAs were significantly up-regulated in PA of CXPA compared to conventional PA, although they are histopathologically similar. Almost all of these transcripts interacted with TP53, a well-known tumor suppressor. In conclusion, we identified differentially expressed miRNAs in PA and CA of CXPA, which were closely associated with TP53 and various cancer-related pathways. We also identified differentially expressed miRNAs in the PA of CXPA and conventional PA which may serve as potential biomarkers.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-17740-9