Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix

• Published in: Scientific reports Vol. 12; no. 1; p. 11192
• Main Authors: Shukla, Shivam, Sah, Amar Nath, Hatiboruah, Diganta, Ahirwar, Shikha, Nath, Pabitra, Pradhan, Asima
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.07.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/67/1517; 639/624/1075/1083; 639/624/1107; 692/308/2778; Brain cancer; Cervical cancer; Cervix; Design; Fabrication; Flavin; Flavin-adenine dinucleotide; Fluorescein; Fluorescence; Fluorescence spectroscopy; Fluorides; Fluorophores; Humanities and Social Sciences; Lasers; Morphology; multidisciplinary; Oral cancer; Porphyrins; Rhodamine; Science; Science (multidisciplinary); Sensors; Smartphones; Spectrum analysis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-15007-x

Fluorescence spectroscopy has the potential to identify discriminatory signatures, crucial for early diagnosis of cervical cancer. We demonstrate here the design, fabrication and testing of a 3D printed smartphone based spectroscopic device. Polarized fluorescence and elastic scattering spectra are captured through the device using a 405 nm laser and a white LED source respectively. The device has been calibrated by comparison of spectra of standard fluorophores (Flavin adenine dinucleotide, fluorescein, rhodamine, and porphyrin) with the corresponding spectra collected from a commercial spectrometer. A few cervical tissue spectra have also been captured for proof of its applicability as a portable, standalone device for the collection of intrinsic fluorescence spectra from human cervix.