Microbiota of Crassostrea virginica larvae during a hatchery crash and under normal production: Amplicon sequence data

• Published in: Data in brief Vol. 40; p. 107755
• Main Authors: Cram, Jacob A., Gray, Matthew W., McFarland, Katherine, Hollins, Ashley
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.02.2022; Elsevier
• Subjects: Amplicon sequences; DADA2 pipeline; Data; Hatchery crash; Oyster larval microbiota; Amplicon sequences; DADA2 pipeline; Hatchery crash; Oyster larval microbiota
• ISSN: 2352-3409; 2352-3409
• DOI: 10.1016/j.dib.2021.107755

We present oyster larval microbiota from two feeding studies, in which wild type and low-salinity tolerant lines were either fed or starved. In one study, all larvae unexpectedly died, which was concurrent with an event in which all larvae in an adjoining oyster hatchery also died. In the other study, no crash occurred in either the study or hatchery. In both cases, larvae were collected and stored frozen, and microbial and host DNA was isolated by phenol-chloroform extraction. Both host 18 s rRNA genes and microbial 16 and 18 s rRNA genes were sequenced using universal primers. We present raw sequences, the pipeline that was used to quantify amplicon sequence variants, and our analysis pipeline that we used to describe how the overall microbial community varied between projects (crashed and non-crashed), feeding status (fed vs not), and strain (wild vs not). These data will be valuable to anyone interested in the microbiota of larval oysters, especially anyone interested in exploring hatchery crashes, effects of starvation, or strain level differences. They also contain a reproducible pipeline of amplicon analysis of host associated microbiota which may serve as a template for other studies. These data are a co-submission to a manuscript submitted to Aquaculture by Matthew grey et al. (2021) entitled Hatchery crashes among shellfish research hatcheries along the Atlantic coast of the United States: a case study at Horn Point Laboratory oyster hatchery. (Manuscript#: AQUACULTURE-D-21–01351R1)