Cell-Cycle-Specific Interaction of Nuclear DNA-Binding Proteins with a CCAAT Sequence from the Human Thymidine Kinase Gene

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 84; no. 23; pp. 8350 - 8354
• Main Authors: Knight, Glenn B., Gudas, Jean M., Pardee, Arthur B.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.12.1987; National Acad Sciences
• Subjects: 3T3 cells; Binding Sites; Binding, Competitive; Biological and medical sciences; Cell Cycle; DNA; DNA Replication; DNA-Binding Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Gene expression; Genes; Humans; Interphase; Methylation; Molecular and cellular biology; Molecular genetics; Nuclear interactions; Nuclear Proteins - metabolism; Nucleoproteins; Oligonucleotides; Oligonucleotides - metabolism; Promoter regions; Promoter Regions, Genetic; Protein Binding; Regulatory Sequences, Nucleic Acid; RNA, Messenger - genetics; Thymidine Kinase - genetics; Transcription, Genetic; Human; Cell culture; Thymidine kinase; Regulation(control); Gene; Transcription; Cell cycle; DNA binding protein; Molecular interaction; Replication; Gene expression
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.84.23.8350

Induction of thymidine kinase parallels the onset of DNA synthesis. To investigate the transcriptional regulation of the thymidine kinase gene, we have examined whether specific nuclear factors interact in a cell-cycle-dependent manner with sequences upstream of this gene. Two inverted CCAAT boxes near the transcriptional initiation sites were observed to form complexes with nuclear DNA-binding proteins. The nature of the complexes changes dramatically as the cells approach DNA synthesis and correlates well with the previously reported transcriptional increase of the thymidine kinase gene.