Housing-temperature reveals energy intake counter-balances energy expenditure in normal-weight, but not diet-induced obese, male mice

Most metabolic studies on mice are performed at room temperature, although under these conditions mice, unlike humans, spend considerable energy to maintain core temperature. Here, we characterize the impact of housing temperature on energy expenditure (EE), energy homeostasis and plasma concentrati...

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Published inCommunications biology Vol. 5; no. 1; p. 946
Main Authors John, Linu Mary, Petersen, Natalia, Gerstenberg, Marina Kjærgaard, Torz, Lola, Pedersen, Kent, Christoffersen, Berit Østergaard, Kuhre, Rune Ehrenreich
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 10.09.2022
Nature Publishing Group
Nature Portfolio
Subjects
64/60
692/163/2743
692/163/2743/393
Animal models
Appetite
Biology
Biomedical and Life Sciences
Body fat
Body weight
Calorimetry
Energy
Energy balance
Energy expenditure
Energy intake
Food intake
Glycerol
High fat diet
Homeostasis
Housing
Life Sciences
Triglycerides
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Summary:Most metabolic studies on mice are performed at room temperature, although under these conditions mice, unlike humans, spend considerable energy to maintain core temperature. Here, we characterize the impact of housing temperature on energy expenditure (EE), energy homeostasis and plasma concentrations of appetite- and glucoregulatory hormones in normal-weight and diet-induced obese (DIO) C57BL/6J mice fed chow or 45% high-fat-diet, respectively. Mice were housed for 33 days at 22, 25, 27.5, and 30 °C in an indirect-calorimetry-system. We show that energy expenditure increases linearly from 30 °C towards 22 °C and is ~30% higher at 22 °C in both mouse models. In normal-weight mice, food intake counter-balances EE. In contrast, DIO mice do not reduce food intake when EE is lowered. By end of study, mice at 30 °C, therefore, had higher body weight, fat mass and plasma glycerol and triglycerides than mice at 22 °C. Dysregulated counterbalancing in DIO mice may result from increased pleasure-based eating. The impact of ambient housing temperature on the interaction of energy intake, energy expenditure and glycemic control in normal and diet-induced obese mice is examined.
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ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-022-03895-8