Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure

Intratracheal bleomycin causes pulmonary injury, inflammation and fibrosis. The characteristic patchy nature of the injury makes analysis challenging. Histological assessment of lung injury is a useful tool to evaluate damage, however quantification is not standardized. We propose a multi-factorial...

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Bibliographic Details
Published inData in brief Vol. 37; p. 107270
Main Authors Golden, Thea, Murray, Alexa, Venosa, Alessandro, Gow, Andrew J.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.08.2021
Elsevier
Subjects
Acute lung injury
Bleomycin injury
Data
Experimental lung Inflammation
Fibrosis
Histological scoring
Acute lung injury
Bleomycin injury
Experimental lung Inflammation
Histological scoring
Fibrosis
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Summary:Intratracheal bleomycin causes pulmonary injury, inflammation and fibrosis. The characteristic patchy nature of the injury makes analysis challenging. Histological assessment of lung injury is a useful tool to evaluate damage, however quantification is not standardized. We propose a multi-factorial approach to assess morphological changes subsequent to intratracheal bleomycin mediated lung injury. Lungs were inflation fixed with paraformaldehyde, sectioned and stained with hematoxylin and eosin. Whole slide images were scanned and ten 400x images were randomly chosen throughout the tissue for further analysis. Using ImageJ software, alveolar wall width was measured, nuclei were counted and airspace was quantified. Morphological changes were identified in mice instilled with bleomycin. This combination offers a robust measure of lung morphology especially in a heterogenous injury.
ISSN:2352-3409
2352-3409
DOI:10.1016/j.dib.2021.107270