Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats

• Published in: Journal of pharmaceutical analysis Vol. 6; no. 6; pp. 389 - 395
• Main Authors: Krishnappa, Pradeepa, Venkatarangaiah, Krishna, Venkatesh, Shimoga Rajanna, Santosh Kumar, Kayattukandy Balan, Rebijith
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.12.2016; Department of Post Graduate Studies and Research in Biotechnology, Kuvempu University, Shankaraghatta 577451, Karnataka, India%Division of Biotechnology, Indian Institute of Horticultural Research IIHR, Bengaluru 560089, India; Xi'an Jiaotong University
• Subjects: (QRPG);Wound; Delonix; Delonix elata; elata;Quercetin-3-rhamnopyranosyl-(1-6);glucopyranoside; healing;Hydroxyproline;Tensile; Hydroxyproline; Original; Quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside (QRPG); strength; Tensile strength; Wound healing; Delonix elata; Wound healing; Quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside (QRPG); Hydroxyproline; Tensile strength
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2016.05.001

Delonix elata L. is a Ceasalpinaceae species and is traditionally used in India for treatment of skin diseases, liverdiseases and rheumatic problems. However, systematic evaluation of its wound healing activity is lacking. Thus,in the present study, we aimed to assess the wound healing activity of D. elata stem bark extract (DSE) and itsisolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside (QRPG) in rats. The formulationseffects on wound healing were assessed by the wound contraction rate, epithelialization period, tensile strength,content of the hydroxyproline, hexosamine and uronic acid in granulation tissue, histopathological studies andCol 1 α (I) expression level in wound tissue by reverse transcription polymerase chain reaction (RT-PCR) study.The topical application of DSE ointment caused faster epithelialization, significant wound contraction (100%),and better tensile strength (710.5 ± 10.5 g/cm2), while QRPG showed wound epithelialization with 98.2%contraction, better than that of the control group (78.18%). The biochemical analysis of granulation tissuerevealed that DSE and QRPG significantly increased hydroxyproline, hexosamine and uronic acid content. Asignificant increase in the expression of Col 1 α (I) was observed in the wound tissue of DSE and QRPG treatedrats. DSE and QRPG were shown to enhance wound healing by increasing collagen synthesis through upregulationof Col 1 α (I), thus validating ethnomedicinal uses.