Different BD BACTEC ™ Blood Culture Bottle Types for the Detection of Fungi in Simulated Sterile Body Fluid Samples

Blood culture systems are a potential alternative to classical cultivation of fungi on mycological media, but there are limited data on the suitability of these systems for culturing other sample types (e.g., sterile body fluids). We conducted a prospective study to evaluate different types of blood...

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Bibliographic Details
Published inDiagnostics (Basel) Vol. 13; no. 10; p. 1699
Main Authors Tomazin, Rok, Pliberšek, Tadej, Oštrbenk Valenčak, Anja, Matos, Tadeja
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 11.05.2023
MDPI
Subjects
Antifungal agents
Aspergillus
BD BACTEC Mycosis-IC/F
BD BACTEC Plus Aerobic/F
Blood
blood cultures
Body fluids
Candida
Fungal infections
Fungi
Laboratories
Medical examination
Morphology
Mycoses
Pathogens
Software
Yeast
France
United States > US
Germany
Aspergillus
BD BACTEC Mycosis-IC/F
blood cultures
Candida
BD BACTEC Plus Aerobic/F
Cryptococcus
fungal infections
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Summary:Blood culture systems are a potential alternative to classical cultivation of fungi on mycological media, but there are limited data on the suitability of these systems for culturing other sample types (e.g., sterile body fluids). We conducted a prospective study to evaluate different types of blood culture (BC) bottles for the detection of different fungal species in non-blood samples. A total of 43 fungal isolates were tested for their ability to grow in BD BACTEC Mycosis-IC/F (Mycosis bottles), BD BACTEC Plus Aerobic/F (Aerobic bottles) and BD BACTEC Plus Anaerobic/F (Anaerobic bottles) (Becton Dickinson, East Rutherford, NJ, USA) BC bottles inoculated with spiked samples without the addition of blood or fastidious organism supplement. Time to detection (TTD) was determined for all BC types tested and compared between groups. In general, Mycosis and Aerobic bottles were similar ( > 0.05). The Anaerobic bottles failed to support growth in >86% of cases. The Mycosis bottles were superior in detecting , spp. and spp. ( < 0.05). The performance of Mycosis and Aerobic bottles was similar, but if cryptococcosis or aspergillosis is suspected, the use of Mycosis bottles is recommended. Anaerobic bottles are not recommended for fungal detection.
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ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics13101699