Inhibitory effects of Carpinus tschonoskii leaves extract on CpG-stimulated pro-inflammatory cytokine production in murine bone marrow-derived macrophages and dendritic cells

• Published in: In vitro cellular & developmental biology. Animal Vol. 48; no. 4; pp. 197 - 202
• Main Authors: Koo, Jung-Eun, Hong, Hye-Jin, Mathema, Vivek Bhakta, Kang, Hee-Kyoung, Hyun, Jin-Won, Kim, Gi-Young, Kim, Young-Ree, Maeng, Young-Hee, Hyun, Chang-Lim, Chang, Weon-Young, Koh, Young-Sang
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.04.2012; Springer Science + Business Media; Society for In Vitro Biology
• Subjects: Animal Genetics and Genomics; Animals; anti-inflammatory activity; Antiinflammatories; Betulaceae - chemistry; Biomedical and Life Sciences; Bone marrow; Cancer; Carpinus; Cell Biology; Cell Culture; Cell lines; Cytokines; Cytokines - metabolism; Dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - metabolism; Developmental Biology; DNA; Epithelial cells; HEK293 Cells; Humans; Immunity, Innate - drug effects; interleukin-12; interleukin-6; kidneys; leaves; Life Sciences; luciferase; Macrophages; Macrophages - drug effects; Macrophages - metabolism; Mice; mitogen-activated protein kinase; NF-kappa B - metabolism; Oligodeoxyribonucleotides - pharmacology; Phosphorylation; Phosphorylation - drug effects; Plant Extracts - pharmacology; Plant Leaves - chemistry; pretreatment; reporter genes; Stem Cells; tumor necrosis factor-alpha; Western blotting; NF-κB; Interleukin; Inflammation
• ISSN: 1071-2690; 1543-706X
• DOI: 10.1007/s11626-012-9495-y

This report describes the anti-inflammatory effects of MeOH extract from leaves of Carpinus tschonoskii (CE) on primary bone marrow-derived macrophage (BMDMs) and dendritic cells (BMDCs). Primary BMDMs and BMDCs were used for pro-inflammatory cytokine production and Western blot analysis. Human embryonic kidney cell line 293 T (HEK293 T) was used to access NF-κB activity. In all cases, CpG DNA was used to stimulate the cells. The CE (0–150 μg/ml) was treated to BMDMs, BMDCs, and HEK293T cells. CE pre-treatment in CpG-stimulated BMDMs and BMDCs showed a dose-dependent inhibitory effect on pro-inflammatory cytokine (e.g., IL-12 p40, IL-6, and TNF-α) production as compared to non-treated controls. The CE pre-treatment had no significant inhibition on mitogen-activated protein kinases (MAPKs) phosphorylation but strongly inhibited IκBα degradation. In NF-κB reporter gene assay, the CE pre-treatment inhibited NF-κB-dependent luciferase activity in a dose-dependent manner. Taken together, these data suggest that CE has significant inhibitory effect on pro-inflammatory cytokine production and warrant further studies concerning potentials of CE for medicinal uses.