An evaluation of the protective effects of chlorogenic acid on broiler chickens in a dextran sodium sulfate model: a preliminary investigation

• Published in: Poultry science Vol. 102; no. 1; p. 102257
• Main Authors: Chen, Yueping, Zha, Pingping, Xu, Hongrui, Zhou, Yanmin
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.01.2023; Elsevier
• Subjects: Animal Feed - analysis; Animals; antioxidant status; broilers; Chickens; Chlorogenic Acid; Dextrans; Diet - veterinary; Dietary Supplements; Glutathione; inflammation; Interferon-gamma; Interleukin-1beta; Interleukin-6; intestinal damage; Male; Malondialdehyde; METABOLISM AND NUTRITION; broilers; inflammation; antioxidant status; chlorogenic acid; intestinal damage
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2022.102257

This study was conducted to investigate the protective effects of chlorogenic acid (CGA) on broilers subjected to dextran sodium sulfate (DSS)-induced intestinal damage. One hundred and forty-four 1-day-old male Arbor Acres broiler chicks were allocated into one of 3 groups with 6 replicates of eight birds each for a 21-d trial. The treatments included: 1) Control group: normal birds fed a basal diet; 2) DSS group: DSS-treated birds fed a basal diet; and 3) CGA group: DSS-treated birds fed a CGA-supplemented control diet. An oral DSS administration via drinking water was performed from 15 to 21 d of age. Compared with the control group, DSS administration reduced 21-d body weight and weight gain from 15 to 21 d, but increased absolute weight of jejunum and absolute and relative weight of ileum (P < 0.05). DSS administration elevated circulating D-lactate concentration and diamine oxidase activity (P < 0.05), which were partially reversed when supplementing CGA (P < 0.05). The oral administration with DSS decreased villus height and villus height/crypt depth ratio, but increased crypt depth in jejunum and ileum (P < 0.05). Compared with the control group, DSS administration increased serum glutathione level and jejunal catalase activity and malonaldehyde accumulation, but decreased jejunal glutathione level (P < 0.05). In contrast, feeding a CGA-supplemented diet normalized serum glutathione and jejunal malonaldehyde levels, and increased jejunal glutathione concentration in DSS-administrated birds (P < 0.05). Additionally, CGA supplementation reduced ileal malonaldehyde accumulation in DSS-treated birds (P < 0.05). DSS challenge increased levels of serum interferon-γ and interleukin-6, jejunal interleukin-1β, tumor necrosis factor-α, and interleukin-6, and ileal interleukin-1β and interleukin-6 when compared with the control group (P < 0.05). The elevated serum interferon-γ and ileal interleukin-6 levels were normalized to control values when supplementing CGA (P < 0.05). The results suggested that CGA administration could partially prevent DSS-induced increased intestinal permeability, oxidative damage, and inflammation in broilers, although it did not improve their growth performance and intestinal morphology.