Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast

• Published in: Bioresource technology Vol. 77; no. 2; pp. 193 - 196
• Main Authors: Hari Krishna, S., Janardhan Reddy, T., Chowdary, G.V.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.2001; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Alkaline H 2O 2 pretreatment; Antigonum leptopus leaves; Biofuel production; Biological and medical sciences; Biotechnology; Carbohydrate Metabolism; Cellulase - metabolism; Cellulose; Cellulose - metabolism; Energy; Enzyme kinetics; Ethanol; Ethanol - economics; Ethanol - metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Glucosidases - chemistry; Glucosidases - metabolism; Industrial applications and implications. Economical aspects; Kluyveromyces - metabolism; Lignin; Lignin - metabolism; Lignocellulose; Plant cell culture; Plant Leaves - chemistry; Poaceae - chemistry; Saccharification; Saccharomyces cerevisiae - metabolism; Simultaneous saccharification and fermentation (SSF); Sugar cane leaves; Temperature; Trichoderma - enzymology; Trichoderma reesei cellulase; Use of agricultural and forest wastes. Biomass use, bioconversion; Yeast; Sugar cane leaves; Trichoderma reesei cellulase; Ethanol; Lignocellulose; Simultaneous saccharification and fermentation (SSF); Antigonum leptopus leaves; Alkaline H 2O 2 pretreatment; Monocotyledones; Yeast; Waste treatment; Biofuel; Biomass; Fungi; Alcoholic fermentation; Gramineae; Dicotyledones; Biotransformation; Angiospermae; Ascomycetes; Bioenergy; Agricultural waste; Pretreatment; Biological treatment; Thallophyta; Enzyme; Polygonaceae; Saccharification; Plant leaf; Lignocellulosics; Saccharum officinarum; Cellulase; Glycosidases; Waste reuse; Microorganism culture; Hydrolases; Spermatophyta; Thermotolerance; O-Glycosidases; Saccharomyces cerevisiae; Kluyveromyces marxianus var. marxianus
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/S0960-8524(00)00151-6

Simultaneous saccharification and fermentation (SSF) studies were carried out to produce ethanol from lignocellulosic wastes (sugar cane leaves and Antigonum leptopus leaves) using Trichoderma reesei cellulase and yeast cells. The ability of a thermotolerant yeast, Kluyveromyces fragilis NCIM 3358, was compared with Saccharomyces cerevisiae NRRL-Y-132. K. fragilis was found to perform better in the SSF process and result in high yields of ethanol (2.5–3.5% w/v) compared to S. cerevisiae (2.0–2.5% w/v). Increased ethanol yields were obtained when the cellulase was supplemented with β-glucosidase. The conversions with K. fragilis were completed in a short time. The substrates were in the following order in terms of fast conversions: Solka floc > A. leptopus > sugar cane.