mTORC1 activity regulates post-translational modifications of glycine decarboxylase to modulate glycine metabolism and tumorigenesis

• Published in: Nature communications Vol. 12; no. 1; p. 4227
• Main Authors: Liu, Rui, Zeng, Lin-Wen, Gong, Rong, Yuan, Fanen, Shu, Hong-Bing, Li, Shu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.07.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13/1; 13/107; 38/1; 38/109; 38/77; 631/45/612/1239; 631/67/2327; 82/1; 82/58; 82/80; Acetylation; Acetyltransferase; Cancer; Catabolism; Deacetylation; Degradation; Enzymatic activity; Enzymes; Glioma; Glycine; Humanities and Social Sciences; Lysine; Metabolism; multidisciplinary; Post-translation; Proteasomes; Pyrimidines; Rapamycin; Science; Science (multidisciplinary); TOR protein; Transcription; Translation; Tumorigenesis; Ubiquitin; Ubiquitin-protein ligase
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-24321-3

Glycine decarboxylase (GLDC) is a key enzyme of glycine cleavage system that converts glycine into one-carbon units. GLDC is commonly up-regulated and plays important roles in many human cancers. Whether and how GLDC is regulated by post-translational modifications is unknown. Here we report that mechanistic target of rapamycin complex 1 (mTORC1) signal inhibits GLDC acetylation at lysine (K) 514 by inducing transcription of the deacetylase sirtuin 3 (SIRT3). Upon inhibition of mTORC1, the acetyltransferase acetyl-CoA acetyltransferase 1 (ACAT1) catalyzes GLDC K514 acetylation. This acetylation of GLDC impairs its enzymatic activity. In addition, this acetylation of GLDC primes for its K33-linked polyubiquitination at K544 by the ubiquitin ligase NF-X1, leading to its degradation by the proteasomal pathway. Finally, we find that GLDC K514 acetylation inhibits glycine catabolism, pyrimidines synthesis and glioma tumorigenesis. Our finding reveals critical roles of post-translational modifications of GLDC in regulation of its enzymatic activity, glycine metabolism and tumorigenesis, and provides potential targets for therapeutics of cancers such as glioma. An increase in glycine decarboxylase (GLDC) activity, a key enzyme for glycine catabolism, has been associated to tumourigenesis. Here, the authors show that mTORC1 activation induces GLDC deacetylation which impairs its ubiquitin-associated degradation leading to increased GLDC activity and tumourigenesis.