Identification of pyroptosis related subtypes and tumor microenvironment infiltration characteristics in breast cancer

• Published in: Scientific reports Vol. 12; no. 1; pp. 10640 - 15
• Main Authors: Huang, Guo, Zhou, Jun, Chen, Juan, Liu, Guowen
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.06.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/250; 631/67/1347; 692/4028; 692/499; Breast cancer; Humanities and Social Sciences; Immunosuppressive agents; Immunotherapy; Infiltration; Medical prognosis; Metastases; multidisciplinary; Mutation; p53 Protein; Patients; Prognosis; Pyroptosis; Science; Science (multidisciplinary); Tumor microenvironment; Tumors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-14897-1

Understanding the association of pyroptosis with tumor progression, prognosis and effect on immunotherapeutic response in breast cancer (BC) is limited. This study analysed forty pyroptosis-related genes to construct the pyroptosis score. Association of the pyroptosis score with the overall survival, clinical features, tumor mutation load, immune cell infiltration, and treatment sensitivity of patients with BC was analysed. Out of 983 BC samples, 304 (30.93%) had genetic alterations with the highest TP53 frequency. We identified three separate subtypes associated with pyroptosis action. These subtypes correlate with the clinicopathological characteristics, TME immune cell infiltration, and disease prognosis. Based on the expression levels of the pyroptosis genes, we divided the pyroptosis score into a high group and a low group. The immune-activated pyroptosis subtype had a higher score with a better prognosis. We also observed that the pyroptosis score correlates with the tumor mutation burden. The pyroptosis score and disease prognosis were directly proportional. A higher pyroptosis score indicated a better prognosis. Results suggest that the pyroptosis-related gene prognosis model is closely related to the immune cell infiltration of BC. The three pyroptosis subtypes associated with BC assist in accurately identifying the tumor subtype, the prognosis of immunotherapy drugs and the patient’s therapeutic response.