Photoautotrophic outdoor two-stage cultivation for oleaginous microalgae Scenedesmus obtusus XJ-15

• Published in: Bioresource technology Vol. 144; pp. 261 - 267
• Main Authors: Xia, Ling, Ge, Hongmei, Zhou, Xuping, Zhang, Delu, Hu, Chunxiang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.09.2013; Elsevier
• Subjects: Autotrophic Processes; Biodiesel; Biofuel production; Biofuels; Biological and medical sciences; Biomass; Biotechnology; Carbon dioxide; Carbon Dioxide - metabolism; Cell Culture Techniques - methods; Culture; Culture Media - pharmacology; Energy; Fatty Acids - biosynthesis; Feasibility Studies; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Lipid induction; Lipids; Lipids - biosynthesis; Microalgae - growth & development; Microalgae - metabolism; Outdoor; Outdoors; Photoautotrophic two-stage cultivation; Photobioreactors - microbiology; Phototrophic Processes; Productivity; Scenedesmaceae; Scenedesmus; Scenedesmus - growth & development; Scenedesmus - metabolism; Scenedesmus obtusus; Scenedesmus obtusus XJ-15; Strategy; Temperature; Time Factors; Scenedesmus obtusus XJ-15; Outdoors; Biodiesel; Lipid induction; Photoautotrophic two-stage cultivation; Phototrophy; Algae; Lipids; Scenedesmus; Autotrophy; Chlorophyceae; Oil; Chlorophyta; Alga; Microorganism; Culture
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.06.112

•Species were screened and evaluated outdoors using tap water.•A new two-stage process was used for S. obtusus XJ-15 to enhance lipid productivity.•Fast cell growth in stage I and lipid induction in stage II were optimized independently.•Higher lipid productivity (60.7mgL−1d−1) and better biodiesel quality were obtained.•Photoautotrophic two-stage process was realized in an outdoor large-scale culture. In this study, Scenedesmus obtusus XJ-15 was firstly selected from seven strains microalgae (Chlorophyta, Scenedesmaceae) and then cultivated using a two-stage strategy, which composed of fast cell growth in stage I and followed by lipid induction in stage II in 5-L flasks outdoors. In stage I, the biomass productivity was increased from 139.4 to 212.1mgL−1d−1. In stage II, lipid content was increased from 26.1% to 47.7% by adding NaCl into the culture. This two-stage process was also realized in an 140-L photobioreactor outdoors, with a biomass productivity of 86.5mgL−1d−1 and CO2 fixation rate of 170.0mgL−1d−1 in the first stage, and high lipid content of 42.1% in the second stage. With such a culture strategy, the overall lipid productivity was improved and better biodiesel quality was obtained. These results suggested the photoautotrophic two-stage system was not only feasible but also effective.