An ethylene biosynthesis enzyme controls quantitative variation in maize ear length and kernel yield

• Published in: Nature communications Vol. 12; no. 1; p. 5832
• Main Authors: Ning, Qiang, Jian, Yinan, Du, Yanfang, Li, Yunfu, Shen, Xiaomeng, Jia, Haitao, Zhao, Ran, Zhan, Jimin, Yang, Fang, Jackson, David, Liu, Lei, Zhang, Zuxin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.10.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 38/23; 38/39; 38/70; 38/71; 38/77; 38/88; 38/91; 42; 42/70; 45; 631/449/1741; 631/449/1870; 631/449/711; Biosynthesis; Cereals; Corn; Crop yield; Enzymes; Ethylene; Fertility; Gene mapping; Genetic diversity; Genetic modification; Genome editing; Grain; Humanities and Social Sciences; Hybrids; Kernels; Meristems; multidisciplinary; Quantitative trait loci; Science; Science (multidisciplinary)
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-26123-z

Maize ear size and kernel number differ among lines, however, little is known about the molecular basis of ear length and its impact on kernel number. Here, we characterize a quantitative trait locus, qEL7 , to identify a maize gene controlling ear length, flower number and fertility. qEL7 encodes 1-aminocyclopropane-1- carboxylate oxidase2 (ACO2), a gene that functions in the final step of ethylene biosynthesis and is expressed in specific domains in developing inflorescences. Confirmation of qEL7 by gene editing of ZmACO2 leads to a reduction in ethylene production in developing ears, and promotes meristem and flower development, resulting in a ~13.4% increase in grain yield per ear in hybrids lines. Our findings suggest that ethylene serves as a key signal in inflorescence development, affecting spikelet number, floral fertility, ear length and kernel number, and also provide a tool to improve grain productivity by optimizing ethylene levels in maize or in other cereals. Considerable genetic variation exists in maize ear size and kernel number. Here the authors show that variation in a gene encoding an ethylene biosynthetic enzyme impacts ear length, flower fertility and kernel yield suggesting an important role for ethylene signaling during inflorescence development.