Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China

• Published in: Poultry science Vol. 101; no. 5; p. 101763
• Main Authors: Yu, Lanping, Liu, Yu, Liu, Mengda, Li, Zixuan, Li, Lin, Wang, Fangkun
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.05.2022; Elsevier
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Chickens; drug resistance gene; Drug Resistance, Bacterial - genetics; Enterococcus faecalis; Enterococcus faecalis - genetics; Gram-Positive Bacterial Infections - epidemiology; Gram-Positive Bacterial Infections - microbiology; Gram-Positive Bacterial Infections - veterinary; Microbial Sensitivity Tests - veterinary; MICROBIOLOGY AND FOOD SAFETY; multilocus sequence typing; multiple drug-resistant; Poultry - microbiology; Virulence - genetics; Enterococcus faecalis; multilocus sequence typing; drug resistance gene; multiple drug-resistant
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2022.101763

Enterococcus faecalis (E. faecalis) is a zoonotic pathogen that causes severe economic losses in the poultry-breeding industry. In our study, cecal samples from broilers with cecal enlargement at slaughterhouses in Tai'an, China, were analyzed. The results revealed that the 61 E. faecalis strains had drug resistance rates ranging from 96.72 to 8.20% against 11 antibiotics in 5 classes, of which erythromycin (96.72%) and tetracycline (96.72%) had the highest rates and vancomycin (8.20%) the lowest. The highest detection rate of multiple drug-resistant strains in 61 isolates was 72.13%. The results of polymerase chain reaction showed that, of the 12 virulence genes, ccf had the highest detection rate (80.33%), followed by asal and cob (both 78.69%), whereas hyl had the lowest (6.56%). Among 15 drug resistance genes, ermB had the highest detection rate (95.08%), followed by tetM (91.80%) and tetL (90.16%), whereas tetK (0.00%) and vanB (0.00%) remained undetected. Of the 34 sequence types found with multilocus sequence typing, the most predominant were ST631 (13.11%, 8/61) and ST634 (8.2%, 5/61). Our results provide a theoretical basis for guiding the rational use of antibiotics and preventing the spread of drug-resistant bacteria, along with epidemiological data for the risk analysis of food-borne bacteria and antimicrobial resistance in poultry farms in Shandong Province.