Monitoring ADP/ATP ratio in yeast cells using the fluorescent-protein reporter PercevalHR

PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used for monitoring energy status of Saccharomyces cerevisiae cells, which are affec...

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Published inBioscience, biotechnology, and biochemistry Vol. 83; no. 5; pp. 824 - 828
Main Authors Nguyen, Phuong Thi Mai, Ishiwata-Kimata, Yuki, Kimata, Yukio
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 04.05.2019
Oxford University Press
Subjects
diauxic shift
Fluorescent reporter: ATP: ADP
yeast
Fluorescent reporter: ATP: ADP
yeast
diauxic shift
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Summary:PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used for monitoring energy status of Saccharomyces cerevisiae cells, which are affected by diauxic shift and mitochondria inhibition, in a non-invasive and non-destructive manner. Yeast cells producing PercevalHR emit green fluorescence, excitation spectrum of which varies dependently on cellular ATP/ATP ratio.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2019.1574204