Enhanced osteoclast-like cell functions on nanophase ceramics

Synthesis of tartrate-resistant acid phosphatase (TRAP) and formation of resorption pits by osteoclast-like cells, the bone-resorbing cells, on nanophase (that is, material formulations with grain sizes less than 100 nm) alumina and hydroxyapatite (HA) were investigated in the present in vitro study...

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Bibliographic Details
Published inBiomaterials Vol. 22; no. 11; pp. 1327 - 1333
Main Authors Webster, Thomas J, Ergun, Celaletdin, Doremus, Robert H, Siegel, Richard W, Bizios, Rena
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.06.2001
Elsevier Science
Subjects
Acid Phosphatase - biosynthesis
Alumina
Animals
Biological and medical sciences
Ceramics
Hydroxyapatite
In Vitro Techniques
Isoenzymes - biosynthesis
Medical sciences
Nanophase
Orthopaedic/dental
Osteoclasts
Osteoclasts - cytology
Osteoclasts - enzymology
Rats
Rats, Wistar
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tartrate-Resistant Acid Phosphatase
Technology. Biomaterials. Equipments
Orthopaedic/dental
Nanophase
Osteoclasts
Hydroxyapatite
Alumina
Grain size
Cell culture
Cell function
Rat
Enzyme
Rodentia
Phosphoric monoester hydrolases
Esterases
Osteoclast
In vitro
Tartrate
Vertebrata
Mammalia
Animal
Acid resistance
Hydrolases
Acid phosphatase
Ceramic materials
Biomedical engineering
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Summary:Synthesis of tartrate-resistant acid phosphatase (TRAP) and formation of resorption pits by osteoclast-like cells, the bone-resorbing cells, on nanophase (that is, material formulations with grain sizes less than 100 nm) alumina and hydroxyapatite (HA) were investigated in the present in vitro study. Compared to conventional (that is, grain sizes larger than 100 nm) ceramics, synthesis of TRAP was significantly greater in osteoclast-like cells cultured on nanophase alumina and on nanophase HA after 10 and 13 days, respectively. In addition, compared to conventional ceramics, formation of resorption pits was significantly greater by osteoclast-like cells cultured on nanophase alumina and on nanophase HA after 7, 10, and 13 days, respectively. The present study, therefore, demonstrated, for the first time, enhanced osteoclast-like cell function on ceramic surfaces with nanometer-size surface topography.
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ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(00)00285-4