Rice MPK17 Plays a Negative Role in the Xa21-Mediated Resistance Against Xanthomonas oryzae pv. oryzae

• Published in: Rice (New York, N.Y.) Vol. 15; no. 1; p. 41
• Main Authors: Zhu, Zheng, Wang, Tianxingzi, Lan, Jinping, Ma, Jinjiao, Xu, Haiqing, Yang, Zexi, Guo, Yalu, Chen, Yue, Zhang, Jianshuo, Dou, Shijuan, Yang, Ming, Li, Liyun, Liu, Guozhen
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2022; Springer Nature B.V; SpringerOpen
• Subjects: Agriculture; Bacterial blight; Biomedical and Life Sciences; Blight; Crop production; Disease resistance; Kinases; Life Sciences; MAP kinase; MAPK; Overexpression; Pathogenesis; Pathogenesis-related proteins; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant Sciences; Protein kinase; Proteins; Resistance gene Xa21; Rice; RNA-mediated interference; Transcription factor; WRKY; Xanthomonas oryzae; WRKY; MAPK; Transcription factor; Resistance gene; Rice; Bacterial blight
• ISSN: 1939-8425; 1939-8433; 1934-8037
• DOI: 10.1186/s12284-022-00590-4

Rice bacterial blight, caused by Xanthomonas oryzae pv . oryzae ( Xoo ), is one of the most serious diseases affecting rice production worldwide. Xa21 was the first disease resistance gene cloned in rice, which encodes a receptor kinase and confers broad resistance against Xoo stains. Dozens of components in the Xa21 -mediated pathway have been identified in the past decades, however, the involvement of mitogen-activated protein kinase (MAPK) genes in the pathway has not been well described. To identify MAPK involved in Xa21 -mediated resistance, the level of MAPK proteins was profiled using Western blot analysis. The abundance of OsMPK17 (MPK17) was found decreased during the rice- Xoo interaction in the background of Xa21 . To investigate the function of MPK17, MPK17-RNAi and over-expression (OX) transgenic lines were generated. The RNAi lines showed an enhanced resistance, while OX lines had impaired resistance against Xoo , indicating that MPK17 plays negative role in Xa21 -mediated resistance. Furthermore, the abundance of transcription factor WRKY62 and pathogenesis-related proteins PR1A were changed in the MPK17 transgenic lines when inoculated with Xoo . We also observed that the MPK17-RNAi and -OX rice plants showed altered agronomic traits, indicating that MPK17 also plays roles in the growth and development. On the basis of the current study and published results, we propose a “ Xa21 -MPK17-WRKY62-PR1A” signaling that functions in the Xa21- mediated disease resistance pathway. The identification of MPK17 advances our understanding of the mechanism underlying Xa21 -mediated immunity, specifically in the mid- and late-stages.