Monocyte Chemoattractant Protein-1 in Obesity and Type 2 Diabetes. Insulin Sensitivity Study

• Published in: Obesity (Silver Spring, Md.) Vol. 15; no. 3; pp. 664 - 672
• Main Authors: Chacón, Matilde R, Fernández-Real, José Manuel, Richart, Cristóbal, Megía, Ana, Gómez, José Manuel, Miranda, Merce, Caubet, Enric, Pastor, Rosa, Masdevall, Carles, Vilarrasa, Núria, Ricard, Wifredo, Vendrell, Joan
• Format: Journal Article
• Language: English
• Published: Oxford, UK The North American Association for the Study of Obesity 01.03.2007; Blackwell Publishing Ltd
• Subjects: adiponectin; adipose tissue; Adult; animal proteins; biomarkers; blood; blood chemistry; Body Mass Index; Case-Control Studies; Chemokine CCL2; Chemokine CCL2 - blood; Chemokine CCL2 - genetics; Chemokine CCL2 - metabolism; cytokines; Diabetes Mellitus, Type 2; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - genetics; Diabetes Mellitus, Type 2 - metabolism; Gene Expression; genetics; Humans; inflammation; insulin resistance; Insulin Resistance - physiology; insulin sensitivity; interleukin-6; Male; men; metabolism; Middle Aged; monocyte chemoattractant protein-1; noninsulin-dependent diabetes mellitus; obesity; Obesity - blood; Obesity - genetics; Obesity - metabolism; physiology; polymerase chain reaction; receptors; RNA, Messenger; RNA, Messenger - metabolism; subcutaneous fat; Subcutaneous Fat - metabolism; tumor necrosis factors; type 2 diabetes; waist-to-hip ratio
• ISSN: 1930-7381; 1930-739X
• DOI: 10.1038/oby.2007.578

OBJECTIVE: Our goal was to test any association between human plasma circulating levels of monocyte chemoattractant protein-1 (cMCP-1) and insulin resistance and to compare monocyte chemoattractant protein-1 (MCP-1) adipose tissue gene expression and cMCP-1 in relation with inflammatory markers. RESEARCH METHODS AND PROCEDURES: cMCP-1 was measured in n = 116 consecutive control male subjects to whom an insulin sensitivity (Si) test was performed. Circulating levels of soluble CD14, soluble tumor necrosis factor receptor type 2 (sTNFR2), soluble interleukin-6 (sIL-6), and adiponectin also were measured. Subcutaneous adipose tissue samples were obtained from n = 107 non-diabetic and type 2 diabetic subjects with different degrees of obesity. Real-time polymerase chain reaction was used to measure gene expression of MCP-1, CD68, tumor necrosis factor-α (TNF-α), and its receptor TNFR2. RESULTS: In the Si study, no independent effect of cMCP-1 levels on insulin sensitivity was observed. In the expression study, in non-diabetic subjects, MCP-1 mRNA had a positive correlation with BMI (r = 0.407, p = 0.003), TNF-α mRNA (r = 0.419, p = 0.002), and TNFR2 mRNA (r = 0.410, p = 0.003). In these subjects, cMCP-1 was found to correlate with waist-to-hip ratio (r = 0.322, p = 0.048). In patients with type 2 diabetes, MCP-1 mRNA was up-regulated compared with non-diabetic subjects. TNF-α mRNA was found to independently contribute to MCP-1 mRNA expression. In this group, CD68 mRNA was found to correlate with BMI (r = 0.455, p = 0.001). DISCUSSION: cMCP-1 is not associated with insulin sensitivity in apparently healthy men. TNF-α is the inflammatory cytokine associated with MCP-1 expression in subcutaneous adipose tissue.