Phenotypic and molecular characterization of recent and archived Erysipelothrix spp. isolated from Brazilian swine

• Published in: Diagnostic microbiology and infectious disease Vol. 69; no. 2; pp. 123 - 129
• Main Authors: Coutinho, Tania A, Imada, Yumiko, Barcellos, David E.S.N, Oliveira, Sergio J, Moreno, Andrea M
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.02.2011; Elsevier
• Subjects: AFLP and PFGE; Amplified Fragment Length Polymorphism Analysis; Animals; Anti-Infective Agents - pharmacology; Antimicrobial susceptibility; Bacteriology; Biological and medical sciences; Brazil; DNA, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field; Erysipelothrix - classification; Erysipelothrix - drug effects; Erysipelothrix - genetics; Erysipelothrix Infections - microbiology; Erysipelothrix spp; Fundamental and applied biological sciences. Psychology; Infectious Disease; Infectious diseases; Internal Medicine; Medical sciences; Microbial Sensitivity Tests; Microbiology; Miscellaneous; Phenotype; Phylogeny; Serotyping; Swine; Brazil; Antimicrobial susceptibility; Swine; Erysipelothrix spp; Serotyping; AFLP and PFGE; Typing; Erysipelothrix; Infection; Vertebrata; Pulsed field electrophoresis; Phenotype; Mammalia; Sensitivity resistance; Bacteria; Artiodactyla; Serotype; Veterinary; Amplified fragment length polymorphism marker; Ungulata
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2010.09.012

Abstract One hundred fifty-one Erysipelothrix spp. isolates from Brazilian swine were characterized by serotyping, determination of antimicrobial susceptibility, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE). Among all isolates, 139 were classified in 18 different serotypes and serotype 2b was the most frequent. The susceptibility profiles of the isolates were very similar among each other, which did not permit subtyping Erysipelothrix spp. isolates by the antimicrobial susceptibility testing. Despite the fact that AFLP and PFGE provided the same discriminatory index (0.98), PFGE was more discriminatory than AFLP, given the types of groups it generates. Regardless the technique employed (AFLP or PFGE), no discrimination between recent and historical isolates was established, neither a fixed epidemiologic pattern for their grouping was observed. Nevertheless, AFLP could be an interesting alternative for discriminating the Erysipelothrix species, while PFGE could be an indication for discerning this bacterium according to the serotypes.