Effect of Additives on Freeze-Drying and Storage of Yarrowia lipolytica Lipase

• Published in: Applied biochemistry and biotechnology Vol. 168; no. 5; pp. 1101 - 1107
• Main Authors: Darvishi, Farshad, Destain, Jacqueline, Nahvi, Iraj, Thonart, Philippe, Zarkesh-Esfahani, Hamid
• Format: Journal Article Web Resource
• Language: English
• Published: New York Springer-Verlag 01.11.2012; Springer; Springer Nature B.V; Humana Press
• Subjects: Additives; Biochemistry; Biological and medical sciences; Bioreactors; Biotechnologie; Biotechnology; Chemistry; Chemistry and Materials Science; Culture Media; Dehydration; Enzymatic activity; Enzyme Stability - drug effects; Enzymes; Freeze Drying; Fundamental and applied biological sciences. Psychology; Glycerol - pharmacology; Hydrogen-Ion Concentration; Life sciences; Lipase - chemistry; Lipase - metabolism; Microbiologie; Microbiology; Polysaccharides - pharmacology; Sciences du vivant; Temperature; Yarrowia - drug effects; Yarrowia - enzymology; Yarrowia lipolytica; Lipase; Formulation; Freeze-drying; Downstream processing; Ascomycota; Freeze drying; Enzyme; Triacylglycerol lipase; Esterases; Carboxylic ester hydrolases; Fungi; Storage; Hydrolases; Yarrowia lipolytica
• ISSN: 0273-2289; 1559-0291; 1559-0291
• DOI: 10.1007/s12010-012-9844-z

The extracellular lipase of Yarrowia lipolytica presents numerous potentialities for biotechnological applications. This work describes the development and storage of powders obtained from supernatants containing Y. lipolytica lipase by freeze-drying as downstream process that is important in obtaining a stable lipase powder with high enzymatic activity. Lipase was produced by Y. lipolytica U6 mutant strain in 20-L bioreactor. Non-concentrated cell-free culture supernatant samples were supplemented with different concentrations (0.5–1 %) of maltodextrin and glycerol as additives to freeze-drying. Effects of additives, temperature, pH, and storage time on lipase powders were determined. After addition of additives, freeze-drying yield increased 3.5-fold compared to supernatant without additive. Maltodextrin with 0.5 % concentration gave the best protection of lipase during dehydration treatment and its freeze-drying yield (77 %) is better than other formulations. Lipase powders were stored at 4 and 25 °C for 46 weeks without loss of lipase activity. A common impediment to the production of commercial enzyme is their low-stability aqueous solutions. The present study shows that freeze-dried lipase powders of Y. lipolytica have good stability for storage and various applications.