Common and unique Arabidopsis proteins involved in stomatal susceptibility to Salmonella enterica and Pseudomonas syringae

• Published in: FEMS microbiology letters Vol. 366; no. 16; pp. 1 - 7
• Main Authors: Rodrigues Oblessuc, Paula, Vaz Bisneta, Mariana, Melotto, Maeli
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.08.2019
• Subjects: Apertures; Apoplast; Arabidopsis; Arabidopsis - genetics; Arabidopsis - microbiology; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Bacteria; Bioassays; Biological Assay; Colonization; Contamination; Genetic Testing; Host-Pathogen Interactions; Leaves; Microbiology; Mutants; Optical Imaging; Pathogens; Physiological aspects; Plant Diseases - microbiology; Plant proteins; Plant Stomata - genetics; Plant Stomata - microbiology; Plants; Pollution prevention; Pseudomonas; Pseudomonas syringae; Pseudomonas syringae - growth & development; Research Letter; Salmonella; Salmonella enterica; Salmonella enterica - growth & development; Stomata; Thermal imaging; Tomatoes; United States; stomatal movement; foodborne illness; phytopathogen; enterobacteria; Arabidopsis mutant screening
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1093/femsle/fnz197

Salmonella enterica is one of the most common pathogens associated with produce outbreaks worldwide; nonetheless, the mechanisms uncovering their interaction with plants are elusive. Previous reports demonstrate that S. enterica ser. Typhimurium (STm), similar to the phytopathogen Pseudomonas syringae pv. tomato (Pst) DC3000, triggers a transient stomatal closure suggesting its ability to overcome this plant defense and colonize the leaf apoplast. In order to discover new molecular players that function in the stomatal reopening by STm and Pst DC3000, we performed an Arabidopsis mutant screening using thermal imaging. Further stomatal bioassay confirmed that the mutant plants exo70h4-3, sce1-3, bbe8, stp1, and lsu2 have smaller stomatal aperture widths than the wild type Col-0 in response to STm 14028s. The mutants bbe8, stp1 and lsu2 have impaired stomatal movement in response to Pst DC3000. These findings indicate that EXO70H4 and SCE1 are involved in bacterial-specific responses, while BBE8, STP1, and LSU2 may be required for stomatal response to a broad range of bacteria. The identification of new molecular components of the guard cell movement induced by bacteria will enable a better understanding of the initial stages of plant colonization and facilitate targeted prevention of leaf contamination with harmful pathogens.