A High-Throughput and Uniform Amplification Method for Cell Spheroids
Cell culture is an important life science technology. Compared with the traditional two-dimensional cell culture, three-dimensional cell culture can simulate the natural environment and structure specificity of cell growth in vivo. As such, it has become a research hotspot. The existing three-dimens...
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| Published in | Micromachines (Basel) Vol. 13; no. 10; p. 1645 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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| Abstract | Cell culture is an important life science technology. Compared with the traditional two-dimensional cell culture, three-dimensional cell culture can simulate the natural environment and structure specificity of cell growth in vivo. As such, it has become a research hotspot. The existing three-dimensional cell culture techniques include the hanging drop method, spinner flask method, etc., making it difficult to ensure uniform morphology of the obtained cell spheroids while performing high-throughput. Here, we report a method for amplifying cell spheroids with the advantages of quickly enlarging the culture scale and obtaining cell spheroids with uniform morphology and a survival rate of over 95%. Technically, it is easy to operate and convenient to change substances. These results indicate that this method has the potential to become a promising approach for cell–cell, cell–stroma, cell–organ mutual interaction research, tissue engineering, and anti-cancer drug screening. |
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| AbstractList | Cell culture is an important life science technology. Compared with the traditional two-dimensional cell culture, three-dimensional cell culture can simulate the natural environment and structure specificity of cell growth in vivo. As such, it has become a research hotspot. The existing three-dimensional cell culture techniques include the hanging drop method, spinner flask method, etc., making it difficult to ensure uniform morphology of the obtained cell spheroids while performing high-throughput. Here, we report a method for amplifying cell spheroids with the advantages of quickly enlarging the culture scale and obtaining cell spheroids with uniform morphology and a survival rate of over 95%. Technically, it is easy to operate and convenient to change substances. These results indicate that this method has the potential to become a promising approach for cell–cell, cell–stroma, cell–organ mutual interaction research, tissue engineering, and anti-cancer drug screening. Cell culture is an important life science technology. Compared with the traditional two-dimensional cell culture, three-dimensional cell culture can simulate the natural environment and structure specificity of cell growth in vivo. As such, it has become a research hotspot. The existing three-dimensional cell culture techniques include the hanging drop method, spinner flask method, etc., making it difficult to ensure uniform morphology of the obtained cell spheroids while performing high-throughput. Here, we report a method for amplifying cell spheroids with the advantages of quickly enlarging the culture scale and obtaining cell spheroids with uniform morphology and a survival rate of over 95%. Technically, it is easy to operate and convenient to change substances. These results indicate that this method has the potential to become a promising approach for cell-cell, cell-stroma, cell-organ mutual interaction research, tissue engineering, and anti-cancer drug screening.Cell culture is an important life science technology. Compared with the traditional two-dimensional cell culture, three-dimensional cell culture can simulate the natural environment and structure specificity of cell growth in vivo. As such, it has become a research hotspot. The existing three-dimensional cell culture techniques include the hanging drop method, spinner flask method, etc., making it difficult to ensure uniform morphology of the obtained cell spheroids while performing high-throughput. Here, we report a method for amplifying cell spheroids with the advantages of quickly enlarging the culture scale and obtaining cell spheroids with uniform morphology and a survival rate of over 95%. Technically, it is easy to operate and convenient to change substances. These results indicate that this method has the potential to become a promising approach for cell-cell, cell-stroma, cell-organ mutual interaction research, tissue engineering, and anti-cancer drug screening. |
| Audience | Academic |
| Author | Huang, Xiaowen Zheng, Chengyun Liu, Liyuan Liu, Xiaoli Liu, Haixia |
| AuthorAffiliation | 1 Department of Hematology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, China 3 Department of Reproductive Medicine, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, China 2 State Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, China |
| AuthorAffiliation_xml | – name: 2 State Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, China – name: 3 Department of Reproductive Medicine, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, China – name: 1 Department of Hematology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, China |
| Author_xml | – sequence: 1 givenname: Liyuan surname: Liu fullname: Liu, Liyuan – sequence: 2 givenname: Haixia orcidid: 0000-0001-9812-3727 surname: Liu fullname: Liu, Haixia – sequence: 3 givenname: Xiaowen orcidid: 0000-0002-1918-1177 surname: Huang fullname: Huang, Xiaowen – sequence: 4 givenname: Xiaoli surname: Liu fullname: Liu, Xiaoli – sequence: 5 givenname: Chengyun surname: Zheng fullname: Zheng, Chengyun |
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| SubjectTerms | 3D cell spheroids Amplification Biocompatibility Cell adhesion & migration Cell culture Culture techniques drug screening high-throughput Life sciences Methods Morphology Signal transduction Silicon wafers Spheres Spheroids Tissue engineering uniform shape |
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| Title | A High-Throughput and Uniform Amplification Method for Cell Spheroids |
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