The Quantitation of Procaine in Equine Plasma by Liquid Chromatography-Linear Ion Trap Mass Spectrometry

A method for the extraction and quantitation of procaine in equine plasma was developed for use with liquid chromatography-mass spectrometry (LC-MS). Procaine was isolated from equine plasma by liquid-liquid extraction at pH 11 with dichloromethane using procaine-d10 as an internal standard. Quantit...

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Bibliographic Details
Published inJournal of analytical toxicology Vol. 31; no. 2; pp. 87 - 92
Main Authors Zientek, Keith D., Anderson, Danielle F., Wegner, Kirsten, Cole, Cynthia
Format Journal Article
LanguageEnglish
Published Niles, IL Oxford University Press 01.03.2007
Preston
Subjects
Analysis
Anesthetics, Local - blood
Anesthetics, Local - pharmacokinetics
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid
Doping in Sports
Female
General pharmacology
Horses
Injections, Subcutaneous
Male
Medical sciences
Nerve Block - methods
Pharmacology. Drug treatments
Procaine - blood
Procaine - pharmacokinetics
Spectrometry, Mass, Electrospray Ionization - methods
Substance Abuse Detection - methods
Biological fluid
Chemical analysis
Liquid liquid extraction
Coupled method
HPLC chromatography
Solvent extraction
Veterinary medicine
Electrospray
Blood
Blood plasma
Local anesthetic
Ester
Vertebrata
Ion trap
Mammalia
Linear configuration
Sample preparation
Animal
Horse
Procaine
Perissodactyla
Mass spectrometry
Ungulata
Quantitative analysis
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Summary:A method for the extraction and quantitation of procaine in equine plasma was developed for use with liquid chromatography-mass spectrometry (LC-MS). Procaine was isolated from equine plasma by liquid-liquid extraction at pH 11 with dichloromethane using procaine-d10 as an internal standard. Quantitation was achieved by LC-MS using a 3-µm C-18 column coupled to an electrospray ionization source on a linear ion-trap mass spectrometer. The limit of detection and limit of quantitation was determined to be 50 and 200 pg/mL, respectively. The lowest limit of detection determined by previous methods was 1 ng/mL. Administration samples were obtained as part of a larger study to determine a regulatory limit for procaine in racehorses and procaine concentrations were determined using this method.
Bibliography:istex:3CDF7CEE8C6E8B50397F26CBEB7A47BC76220D45
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ObjectType-Article-1
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ISSN:0146-4760
1945-2403
DOI:10.1093/jat/31.2.87