Two Chalcone Synthase Isozymes Participate Redundantly in UV-Induced Sakuranetin Synthesis in Rice

• Published in: International journal of molecular sciences Vol. 21; no. 11; p. 3777
• Main Authors: Park, Hye Lin, Yoo, Youngchul, Bhoo, Seong Hee, Lee, Tae-Hoon, Lee, Sang-Won, Cho, Man-Ho
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 27.05.2020; MDPI AG
• Subjects: Acyltransferases - chemistry; Acyltransferases - genetics; Acyltransferases - metabolism; Amino Acid Motifs; Amino Acid Sequence; chalcone synthase; Conserved Sequence; Flavonoids - biosynthesis; Gene Expression Regulation, Plant; Isoenzymes - metabolism; Kinetics; Multigene Family; Mutation - genetics; Oryza - enzymology; Oryza - genetics; Oryza - radiation effects; Phylogeny; phytoalexin; Plant Proteins - genetics; Plant Proteins - metabolism; Recombinant Proteins - metabolism; rice; sakuranetin; sakuranetin biosynthesis; Ultraviolet Rays; UV; chalcone synthase; rice; sakuranetin; sakuranetin biosynthesis; phytoalexin
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21113777

Chalcone synthase (CHS) is a key enzyme in the flavonoid pathway, participating in the production of phenolic phytoalexins. The rice genome contains 31 family genes ( s). The molecular characterization of s suggests that and belong in the bona fide CHSs, while the other members are categorized in the non-CHS group of type III polyketide synthases (PKSs). Biochemical analyses of recombinant OsCHSs also showed that OsCHS24 and OsCHS8 catalyze the formation of naringenin chalcone from -coumaroyl-CoA and malonyl-CoA, while the other OsCHSs had no detectable CHS activity. OsCHS24 is kinetically more efficient than OsCHS8. Of the s, also showed the highest expression levels in different tissues and developmental stages, suggesting that it is the major CHS isoform in rice. In mutant leaves, sakuranetin content decreased to 64.6% and 80.2% of those in wild-type leaves at 2 and 4 days after UV irradiation, respectively, even though expression was mostly suppressed. Instead, the expression was markedly increased in the mutant under UV stress conditions compared to that in the wild-type, which likely supports the UV-induced production of sakuranetin in . These results suggest that OsCHS24 acts as the main CHS isozyme and OsCHS8 redundantly contributes to the UV-induced production of sakuranetin in rice leaves.