Leptomonas seymouri and Crithidia fasciculata exoantigens can discriminate human cases of visceral leishmaniasis from American tegumentary leishmaniasis ones

• Published in: Revista do Instituto de Medicina Tropical de São Paulo Vol. 59; pp. e1 - 4
• Main Authors: Kesper, Norival, Teixeira, Marta Maria G, Lindoso, José Angelo L, Barbieri, Clara Lúcia, Umezawa, Eufrosina Setsu
• Format: Journal Article
• Language: English
• Published: Brazil Instituto de Medicina Tropical de Sao Paulo 01.01.2017; Instituto de Medicina Tropical; Universidade de São Paulo (USP)
• Subjects: Antibodies, Protozoan - blood; Antigens; Antigens, Protozoan - blood; Brief Communication; Crithidia fasciculata; Crithidia fasciculata - immunology; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Exoantigens; Humans; Immunoassay; Immunoglobulin G - blood; Leishmania (Leishmania) infantum chagasi; Leishmania donovani - immunology; Leishmaniasis; Leishmaniasis, Cutaneous - diagnosis; Leishmaniasis, Cutaneous - parasitology; Leishmaniasis, Visceral - diagnosis; Leishmaniasis, Visceral - parasitology; Leptomonas seymouri; Medical diagnosis; Parasites; Parasitic diseases; TROPICAL MEDICINE; Trypanosomatina - immunology; Crithidia fasciculata; Leishmaniasis; Leptomonas seymouri; Leishmania (Leishmania) infantum chagasi; Exoantigens
• ISSN: 0036-4665; 1678-9946; 1678-9946; 0036-4665
• DOI: 10.1590/S1678-9946201759001

Exoantigens (exo) from Leptomonas seymouri and Crithidia fasciculata were used in an enzyme linked immunosorbent assay (ELISA), showing 100% reactivity with sera from visceral leishmaniasis (VL) cases, and no reactivity with American tegumentary leishmaniasis (ATL) ones. Our results have indicated that these exoantigens can be applied in the discrimination of VL and ATL cases.