Characterization of a Protein, Released by the T47D Cell Line, Immunologically Related to the Major Envelope Protein of Mouse Mammary Tumor Virus

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 82; no. 5; pp. 1531 - 1535
• Main Authors: Segev, Nava, Hizi, Amnon, Kirenberg, Fernand, Keydar, Iafa
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.03.1985; National Acad Sciences
• Subjects: Adenocarcinoma - immunology; Adenocarcinoma - microbiology; Animals; Antibodies; Antigens; Biological and medical sciences; Breast Neoplasms - immunology; Breast Neoplasms - microbiology; Carcinoma; Cell Line; Cell lines; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Cross Reactions; Female; Fundamental and applied biological sciences. Psychology; Gels; glycoproteins; Glycoproteins - immunology; Humans; Immunology; Isoelectric Point; mammary tumor virus; Mammary Tumor Virus, Mouse - immunology; Mice; Molecular and cellular biology; Molecular Weight; proteins; Tumor cell line; Viral Envelope Proteins - immunology; Virions; Viruses; Adenocarcinoma; Human; Antigenic relationship; Oncovirinae; Retroviridae; Carcinogenesis; Virus; Characterization; Proteins; Cell line; Antigenicity; Etiology; Oncogenic virus; Type B oncovirus; Mammary gland; Mouse mammary tumor virus; Virus envelope
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.82.5.1531

The T47D human mammary adenocarcinoma cell line in vitro releases viral particles as well as soluble proteins. Both fractions were shown to contain antigens that immunologically crossreact with the major glycoprotein (gp52) of mouse mammary tumor virus. The crossreacting antigens are located on polypeptides with apparent molecular weights of about 68,000 and 60,000. The larger one is present in viral particles whereas both are found in the soluble fraction. Both proteins are glycosylated. The human tissue culture proteins differ from gp52 not only in molecular weight but also in charge heterogeneity and in polypeptide profiles obtained after partial proteolysis. The results suggest that there is a restricted similarity between MMTV gp52 and the immunologically related T47D proteins.