N-acetylated Proline–Glycine–Proline induced G-protein dependent chemotaxis of neutrophils is independent of CXCL8 release

• Published in: European journal of pharmacology Vol. 668; no. 3; pp. 428 - 434
• Main Authors: Overbeek, Saskia A., Henricks, Paul A.J., Srienc, Anja I., Koelink, Pim J., de Kruijf, Petra, Lim, Herman D., Smit, Martine J., Zaman, Guido J.R., Garssen, Johan, Nijkamp, Frans P., Kraneveld, Aletta D., Folkerts, Gert
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.10.2011; Elsevier
• Subjects: antagonists; Antibodies - immunology; Biological and medical sciences; calcium; Calcium - metabolism; Chemotaxis; Chemotaxis, Leukocyte - drug effects; Chronic airway inflammation; collagen; Collagen - metabolism; CXCL8; G-proteins; Heterotrimeric GTP-Binding Proteins - metabolism; Humans; Imidazoles - chemistry; Imidazoles - pharmacology; inflammation; Interleukin-8 - antagonists & inhibitors; Interleukin-8 - immunology; Interleukin-8 - secretion; mechanism of action; Medical sciences; N-ac-PGP; Neutrophil; neutrophils; Neutrophils - cytology; Neutrophils - drug effects; Neutrophils - metabolism; Neutrophils - secretion; Oligopeptides - pharmacology; Peptide Fragments - pharmacology; pertussis toxin; Pertussis Toxin - toxicity; pharmacology; Pharmacology. Drug treatments; pneumonia; receptors; Receptors, G-Protein-Coupled - metabolism; Receptors, Interleukin-8B - metabolism; CXCL8; Neutrophil; Chronic airway inflammation; Chemotaxis; N-ac-PGP; Proline; Inflammation; Glycine; Respiratory system; Respiratory tract; Chronic; Aminoacid; Neurotransmitter; Release; G protein
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2011.03.022

Chronic inflammation in lung diseases contributes to lung tissue destruction leading to the formation of chemotactic collagen fragments such as N-acetylated Proline–Glycine–Proline (N-ac-PGP). In this study, we investigated in more detail the mechanism of action of N-ac-PGP in neutrophilic inflammation. N-ac-PGP was chemotactic for human neutrophils via pertussis toxin sensitive G protein-coupled receptors in vitro and directly activated this cell type, which led to cytosolic calcium mobilization and release of CXCL8. Furthermore, using a selective CXCR2 antagonist confirmed that N-ac-PGP-induced neutrophil chemotaxis is mediated through CXCR2 activation. To determine whether N-ac-PGP was solely responsible for the migration and activation of human neutrophils in vitro and not the released CXCL8 upon stimulation with N-ac-PGP, an antibody directed against CXCL8 was used. Performing chemotaxis and calcium influx assays in the presence of this antibody did not alter the effects of N-ac-PGP whereas effects of CXCL8 were attenuated. These experiments indicate that N-ac-PGP, in addition to the direct induction of chemotaxis, also directly activates neutrophils to release CXCL8. In vivo, this may lead in the long term to a self-maintaining situation enhanced by both N-ac-PGP and CXCL8, leading to a further increase in neutrophil infiltration and chronic inflammation.