Superresolution Imaging of Albumin-Conjugated Fluorescent Nanodiamonds in Cells by Stimulated Emission Depletion

A bunch of loners: Fluorescent nanodiamonds (FNDs) noncovalently conjugated with bovine serum albumin (BSA) or α‐lactalbumin exhibited good dispersibility in a buffer with only minor or no agglomeration. They are useful as photostable fluorescent markers in cells for superresolution imaging by STED...

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Published inAngewandte Chemie (International ed.) Vol. 50; no. 10; pp. 2262 - 2265
Main Authors Tzeng, Yan-Kai, Faklaris, Orestis, Chang, Be-Ming, Kuo, Yung, Hsu, Jui-Hung, Chang, Huan-Cheng
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.03.2011
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
Animals
Cattle
Endocytosis
fluorescence
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Lactalbumin - chemistry
Microscopy, Confocal
Microscopy, Fluorescence
nanodiamond
Nanodiamonds - chemistry
nanoparticles
Serum Albumin, Bovine - chemistry
STED microscopy
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Summary:A bunch of loners: Fluorescent nanodiamonds (FNDs) noncovalently conjugated with bovine serum albumin (BSA) or α‐lactalbumin exhibited good dispersibility in a buffer with only minor or no agglomeration. They are useful as photostable fluorescent markers in cells for superresolution imaging by STED (see confocal fluorescence image of an FND‐labeled cell (left) and STED image of single BSA‐conjugated FNDs (right)).
Bibliography:National Science Council, Taiwan - No. 99-2119M-001-026-
ark:/67375/WNG-QJHTGW3Q-V
istex:11253E90B8E3818C98631E20AF0D9997AD78E946
This research was supported by the Academia Sinica and the National Science Council, Taiwan with Grant No. 99-2119M-001-026-. We thank Dr. K. Y. Han and Dr. C. Eggeling at the Max Planck Institute for Biophysical Chemistry in Göttingen (Germany) for instruction and assistance in setting up the STED system.
ArticleID:ANIE201007215
Academia Sinica
These authors contributed equally.
This research was supported by the Academia Sinica and the National Science Council, Taiwan with Grant No. 99‐2119M‐001‐026‐. We thank Dr. K. Y. Han and Dr. C. Eggeling at the Max Planck Institute for Biophysical Chemistry in Göttingen (Germany) for instruction and assistance in setting up the STED system.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201007215