Identification of a novel circulating recombinant form (CRF33_01B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia

A molecular epidemiological investigation was conducted among various risk populations (n = 184) in Kuala Lumpur, Malaysia, in 2003 to 2005, on the basis of nucleotide sequences of protease and reverse transcriptase regions. In addition to circulating HIV-1 strains, including CRF01_AE (57.1%), subty...

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Published inJournal of acquired immune deficiency syndromes (1999) Vol. 43; no. 5; p. 523
Main Authors Tee, Kok Keng, Li, Xiao-Jie, Nohtomi, Kyoko, Ng, Kee Peng, Kamarulzaman, Adeeba, Takebe, Yutaka
Format Journal Article
LanguageEnglish
Published United States 15.12.2006
Subjects
Adult
Aged
Base Sequence
Child
Child, Preschool
Female
Genotype
HIV Infections - epidemiology
HIV Infections - virology
HIV-1 - classification
HIV-1 - genetics
Humans
Malaysia - epidemiology
Male
Middle Aged
Molecular Epidemiology
Molecular Sequence Data
Phylogeny
Malaysia
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Summary:A molecular epidemiological investigation was conducted among various risk populations (n = 184) in Kuala Lumpur, Malaysia, in 2003 to 2005, on the basis of nucleotide sequences of protease and reverse transcriptase regions. In addition to circulating HIV-1 strains, including CRF01_AE (57.1%), subtype B (20.1%), and subtype C (0.5%), we detected a candidate with a new circulating recombinant form (CRF). We determined four near-full-length nucleotide sequences with identical subtype structure from epidemiologically unlinked individuals of different risk and ethnic groups. In this chimera, two short subtype B segments were inserted into the gag-RT region in a backbone of CRF01_AE. The recombinant structure was distinct from previously identified CRF15_01B in Thailand. In agreement with the current HIV nomenclature system, this constitutes a novel CRF (CRF33_01B). The overall prevalence of CRF33_01B is 19.0% (35/184). Although the prevalence of CRF33_01B is particularly high among injecting drug users (42.0%, 21/50), it is also detected in a substantial proportion of homo-/bisexual males (18.8%, 3/16) and heterosexuals (9.8%, 9/92). Moreover, unique recombinant forms composed of CRF01_AE and subtype B that have a significant structural relationship with CRF33_01B were detected in 1.6% (3/184) of study subjects, suggesting an ongoing recombination process in Malaysia. This new CRF seems to be bridging viral transmission between different risk populations in this country.
ISSN:1525-4135
DOI:10.1097/01.qai.0000242451.74779.a7