Differentially expressed genes related to symbiotic association in a supernodulating soybean mutant and its wild-type

• Published in: Molecular plant pathology Vol. 7; no. 4; pp. 235 - 247
• Main Authors: LESTARI, PUJI, VAN, KYUJUNG, KIM, MOON YOUNG, HWANG, CHEOL HO, LEE, BYUN-WOO, LEE, SUK-HA
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.07.2006; Blackwell
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Bradyrhizobium japonicum; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Glycine max; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Symbiotic relation; Bradyrhizobium japonicum; Nodulation; Gene expression; Symbiont; Glycine max; Grain legume; Leguminosae; Dicotyledones; Angiospermae; Bacteria; Spermatophyta; Rhizobiaceae; Mutation
• ISSN: 1464-6722; 1364-3703
• DOI: 10.1111/j.1364-3703.2006.00334.x

SUMMARY To understand the molecular basis of symbiotic association, a cDNA‐AFLP technique was used to identify differentially expressed transcripts between a supernodulating soybean mutant, SS2‐2, and its wild‐type, Sinpaldalkong 2. As sources of cDNA‐AFLP templates, trifoliates of 2‐week‐old plants were collected 1 week after Bradyrhizobium japonicum inoculation. A total of 147 bands out of 4000 amplicons were recognized as differentially expressed fragments, with 40 transcript‐derived fragments (TDFs) in SS2‐2 and 65 TDFs in Sinpaldalkong 2. Qualitative and quantitative real‐time RT‐PCR assays suggested that the expression patterns of genes in both genotypes were clearly differentiated. TDFs homologous to nodulin (65S2) and a putative senescence‐associated protein (9S1) were up‐regulated in SS2‐2, whereas Sinpaldalkong 2 showed up‐regulation of a receptor‐like kinase (48sin1) and a kinase‐like protein (17sin1). This indicates that different genes may be involved in regulation of the symbiotic programme that distinguishes SS2‐2 from its wild‐type. A TDF showing a change in a single base from A (Sinpaldalkong 2) to T (SS2‐2) in this study was identified as a Glycine max nodule autoregulation receptor‐like protein kinase precursor, previously identified by map‐based cloning. These results demonstrate that cDNA‐AFLP is a powerful technique to detect interesting genes without prior assumptions about the nature of the genes. The differentially expressed genes between Sinpaldalkong 2 and SS2‐2 suggest that different signal transduction pathways for symbiosis may be involved in the two soybean genotypes.