The Evaluation and Optimization of Intraoperative Touch Imprint Cytology for Sentinel Lymph Nodes in Early-stage Breast Cancer in China

• Published in: World journal of surgery Vol. 34; no. 10; pp. 2325 - 2332
• Main Authors: Chen, Jia-jian, Yang, Ben-long, Zhang, Jia-xin, Xu, Wei-ping, Shao, Zhi-min, Wu, Jiong
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.10.2010; Springer‐Verlag; Springer; Springer Nature B.V
• Subjects: Abdominal Surgery; Adult; Aged; Aged, 80 and over; Axillary Lymph Node Dissection; Biological and medical sciences; Breast Neoplasms - pathology; Carcinoma, Ductal, Breast - pathology; Carcinoma, Intraductal, Noninfiltrating - pathology; Cardiac Surgery; China; Core Needle Biopsy; Female; Freeze Section; General aspects; General Surgery; Gynecology. Andrology. Obstetrics; Histocytological Preparation Techniques - methods; Humans; Intraoperative Period; Lymph Nodes - pathology; Lymphatic Metastasis; Mammary gland diseases; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Neoplasm Staging; Sentinel Lymph Node; Sentinel Lymph Node Biopsy; Surgery; Thoracic Surgery; Tumors; Vascular Surgery; Young Adult; Asia; China; Sentinel Lymph Node; Freeze Section; Sentinel Lymph Node Biopsy; Axillary Lymph Node Dissection; Core Needle Biopsy; Evaluation; Breast disease; Sentinel lymph node; Cytology; Intraoperative; Early stage; Breast cancer; Malignant tumor; Optimization; Genomic imprinting; Medicine; Mammary gland diseases; Tactile sensitivity; Treatment; Surgery; Mammary gland; Cancer
• ISSN: 0364-2313; 1432-2323
• DOI: 10.1007/s00268-010-0684-0

Background Accurate intraoperative diagnosis of sentinel lymph node (SLN) metastasis reduces the need for additional surgery in patients with involved nodes. The present study evaluates the clinical value of multiple cross-sectional touch imprint cytology (TIC) as an intraoperative assessment for the diagnosis of SLN metastasis. Methods This study consisted of 366 patients with surgically harvested SLNs that were sliced along their long axis at 2.0–3.0-mm intervals and 122 patients with SLNs that were sliced along their short axis at 1.5-mm intervals using a cutting apparatus designed by our group. The first group of patients was enrolled in this study between February 2005 and February 2008, while the second group was enrolled between March 2008 and January 2009. Serial sectioning of the SLNs at 100-μm intervals with hematoxylin-eosin (H&E) staining was used as the gold standard for pathological diagnosis. Results Multiple cross-sectional TIC has a sensitivity, specificity, and overall accuracy rate of 92.0, 99.0, and 97.5%, respectively, on a per-patient basis, and it is superior to the standard imprint preparation protocol. Furthermore, the multiple cross-sectional TIC technique developed in this study was observed to detect more accurately macrometastases on a per-patient basis in comparison to the typical protocol ( P  = 0.023). Of the patients included in this study, 97.7% had a positive SLN within their first three harvested SLNs. Conclusions Multiple cross-sectional TIC is superior to the standard protocol, especially due to its ability to locate macrometastasis. Limiting intraoperative TIC to the first three harvested SLNs in the diagnosis of SLN metastasis may make this diagnostic procedure significantly cheaper and easier for pathologists to perform.