Monitoring the purification by high‐performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase‐sensitive two‐dimensional nuclear magnetic resonance

High‐resolution phase‐sensitive two‐dimensional proton nuclear magnetic resonance was used to monitor the preparation by high‐performance liquid chromatography of homogeneous proteins from the venom of Naja mossambica mossambica. This resulted in the characterization of a heterogeneous protein prepa...

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Bibliographic Details
Published inEuropean Journal of Biochemistry Vol. 168; no. 3; pp. 603 - 607
Main Authors OTTING, Gottfried, MARCHOT, Pascale, BOUGIS, Pierre E., ROCHAT, Hervé, WÜTHRICH, Kurt
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 02.11.1987
Blackwell
Wiley
Subjects
Amino Acids - analysis
Animal biology
Animals
Applied sciences
cardiotoxins
Chromatography, High Pressure Liquid
Exact sciences and technology
Heart - drug effects
Life Sciences
Magnetic Resonance Spectroscopy
Naja mossambica mossambica
Other techniques and industries
Protein Conformation
Proteins - isolation & purification
Snake Venoms - analysis
venom
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Summary:High‐resolution phase‐sensitive two‐dimensional proton nuclear magnetic resonance was used to monitor the preparation by high‐performance liquid chromatography of homogeneous proteins from the venom of Naja mossambica mossambica. This resulted in the characterization of a heterogeneous protein preparation VII2, which had been used in earlier structural studies by NMR, as well as a homogeneous protein CTXIIb and a nearly homogeneous protein fraction CTXIIa, which are now both subject to further investigations of their solution conformations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-2956
1432-1033
1432-1327
DOI:10.1111/j.1432-1033.1987.tb13459.x