High expression of ubiquitin carboxy‐terminal hydrolase‐L1 and ‐L3 mRNA predicts early recurrence in patients with invasive breast cancer

• Published in: Cancer science Vol. 97; no. 6; pp. 523 - 529
• Main Authors: Miyoshi, Yasuo, Nakayama, Satoshi, Torikoshi, Yasuhiro, Tanaka, Satoshi, Ishihara, Hideki, Taguchi, Tetsuya, Tamaki, Yasuhiro, Noguchi, Shinzaburo
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 01.06.2006; Blackwell
• Subjects: Antigens, CD - metabolism; Biological and medical sciences; Biomarkers, Tumor - analysis; Breast Neoplasms - enzymology; Breast Neoplasms - pathology; Female; Gynecology. Andrology. Obstetrics; Humans; Mammary gland diseases; Medical sciences; Neoplasm Recurrence, Local - metabolism; Neoplasm Recurrence, Local - pathology; Original; Postmenopause; Premenopause; Prognosis; Receptors, Estrogen - metabolism; Receptors, Progesterone - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Tumors; Ubiquitin Thiolesterase - biosynthesis; Human; Relapse; Prognosis; Enzyme; Isozyme; Breast cancer; Malignant tumor; Infiltrating tumor; Gene expression; Mammary gland diseases; Cancerology; Messenger RNA; Genetics; Hydrolases; Early
• ISSN: 1347-9032; 1349-7006
• DOI: 10.1111/j.1349-7006.2006.00202.x

The present study investigated the mRNA expression level of ubiquitin c‐terminal hydrolase (UCH)‐L1 and ‐L3 in breast cancer tissue and aimed to elucidate its association with tumor characteristics and patient prognosis. UCH‐L1 and UCH‐L3 mRNA levels in invasive breast cancer (n = 100) were determined by a real‐time polymerase chain reaction (PCR) assay and their relationship with various clinicopathological characteristics of breast tumors as well as patient prognosis were studied. UCH‐L3 mRNA level was significantly upregulated in breast cancer tissue compared to adjacent normal breast tissue (P < 0.005), and UHC‐L1 mRNA level also showed a non‐significant increase in tumor tissue compared to adjacent normal breast tissue. Both UCH‐L1 and UCH‐L3 mRNA levels were significantly higher in high histological grade tumors than in low histological grade tumors (P < 0.001 and P < 0.005, respectively). High UCH‐L1 mRNA level was significantly associated with negative estrogen receptor status (P < 0.05) and negative progesterone receptor status (P < 0.05). Patients with both UCH‐L1 and UCH‐L3 mRNA high tumors showed a significantly poorer prognosis than those in the UCH‐L1 or UCH‐L3 mRNA low group (P < 0.005). These observations that UCH‐L3 mRNA level is upregulated in breast cancer tissue, and breast tumors with both UCH‐L1 and UCH‐L3 mRNA high expression are associated with a poor prognosis, suggest the possible involvement of UCH‐L1 and UCH‐L3 in the pathogenesis and progression of breast cancer. (Cancer Sci 2006; 97: 523– 529)