Crosslinking Strategies for 3D Bioprinting of Polymeric Hydrogels

• Published in: Small (Weinheim an der Bergstrasse, Germany) Vol. 16; no. 35; pp. e2002931 - n/a
• Main Authors: GhavamiNejad, Amin, Ashammakhi, Nureddin, Wu, Xiao Yu, Khademhosseini, Ali
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.09.2020
• Subjects: 3-D printers; 3D bioprinting; Bioengineering; bioinks; Biomechanics; Biomimetics; Bioprinting; Crosslinking; crosslinking strategies; Design modifications; Hydrogels; hydrogel–cell interactions; Nanotechnology; Printing, Three-Dimensional; Three dimensional printing; Tissue Engineering; bioinks; tissue engineering; 3D bioprinting; crosslinking strategies; hydrogel-cell interactions
• ISSN: 1613-6810; 1613-6829; 1613-6829
• DOI: 10.1002/smll.202002931

Three‐dimensional (3D) bioprinting has recently advanced as an important tool to produce viable constructs that can be used for regenerative purposes or as tissue models. To develop biomimetic and sustainable 3D constructs, several important processing aspects need to be considered, among which crosslinking is most important for achieving desirable biomechanical stability of printed structures, which is reflected in subsequent behavior and use of these constructs. In this work, crosslinking methods used in 3D bioprinting studies are reviewed, parameters that affect bioink chemistry are discussed, and the potential toward improving crosslinking outcomes and construct performance is highlighted. Furthermore, current challenges and future prospects are discussed. Due to the direct connection between crosslinking methods and properties of 3D bioprinted structures, this Review can provide a basis for developing necessary modifications to the design and manufacturing process of advanced tissue‐like constructs in future. When designing a 3D bioprinting system, the selection of an appropriate crosslinking method is required, to enable successful printability and to ensure cytocompatibility, stability, and sustainability of the resulting tissue constructs. The present work systematically summarizes recent advances made in the development of crosslinking methods and their application in 3D bioprinting.