Laser speckle imaging based on photothermally driven convection

Laser speckle imaging (LSI) is an interferometric technique that provides information about the relative speed of moving scatterers in a sample. Photothermal LSI overcomes limitations in depth resolution faced by conventional LSI by incorporating an excitation pulse to target absorption by hemoglobi...

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Bibliographic Details
Published inJournal of biomedical optics Vol. 21; no. 2; p. 026011
Main Authors Regan, Caitlin, Choi, Bernard
Format Journal Article
LanguageEnglish
Published United States Society of Photo-Optical Instrumentation Engineers 01.02.2016
Subjects
Animals
Blood Physiological Phenomena
Convection
Image Processing, Computer-Assisted - methods
Interferometry - methods
Lasers
Phantoms, Imaging
Rabbits
Research Papers: Imaging
Temperature
Titanium
convection
photothermal
laser speckle imaging
mechanism
blood
expansion
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Summary:Laser speckle imaging (LSI) is an interferometric technique that provides information about the relative speed of moving scatterers in a sample. Photothermal LSI overcomes limitations in depth resolution faced by conventional LSI by incorporating an excitation pulse to target absorption by hemoglobin within the vascular network. Here we present results from experiments designed to determine the mechanism by which photothermal LSI decreases speckle contrast. We measured the impact of mechanical properties on speckle contrast, as well as the spatiotemporal temperature dynamics and bulk convective motion occurring during photothermal LSI. Our collective data strongly support the hypothesis that photothermal LSI achieves a transient reduction in speckle contrast due to bulk motion associated with thermally driven convection. The ability of photothermal LSI to image structures below a scattering medium may have important preclinical and clinical applications.
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ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.21.2.026011