Age-related base excision repair activity in mouse brain and liver nuclear extracts

To assess DNA repair activity relative to age, in vitro base excision repair assays were performed using brain and liver nuclear extracts prepared from mice of various ages. An 85% decline in repair activity was observed in brain nuclear extracts and a 50% decrease in liver nuclear extracts prepared...

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Published inThe journals of gerontology. Series A, Biological sciences and medical sciences Vol. 58; no. 3; p. 205
Main Authors Intano, Gabriel W, Cho, Eun Ju, McMahan, C Alex, Walter, Christi A
Format Journal Article
LanguageEnglish
Published United States 01.03.2003
Subjects
Aging - genetics
Aging - metabolism
Animals
Blotting, Western
Brain - metabolism
Cell Nucleus - metabolism
DNA Glycosylases
DNA Ligases - pharmacology
DNA Polymerase beta - metabolism
DNA Repair
Liver - metabolism
Male
Mice
Mice, Inbred Strains
N-Glycosyl Hydrolases - metabolism
Uracil-DNA Glycosidase
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Summary:To assess DNA repair activity relative to age, in vitro base excision repair assays were performed using brain and liver nuclear extracts prepared from mice of various ages. An 85% decline in repair activity was observed in brain nuclear extracts and a 50% decrease in liver nuclear extracts prepared from old mice compared with 6-day-old mice. Brain nuclear extracts prepared from old mice showed a decreased abundance of DNA polymerase-beta, but the addition of purified protein did not restore base excision repair activity. Abundances of other tested base excision repair proteins did not change relative to age. The conclusion is that, during aging, a decline in DNA repair could contribute to increased levels of DNA damage and mutagenesis.
ISSN:1079-5006
DOI:10.1093/gerona/58.3.b205