A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species

A PCR-RFLP method targeted toward 26S rDNA and with 2 restriction enzymes, CfoI and BstF51, was developed to identify 11 Malassezia species. Not only type and standard strains but also 13 clinical isolates were identified successfully in this study. The results of identifications were confirmed by D...

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Bibliographic Details
Published inJournal of microbiological methods Vol. 61; no. 2; pp. 281 - 284
Main Authors Mirhendi, Hossein, Makimura, Koichi, Zomorodian, Kamiar, Yamada, Tsuyoshi, Sugita, Takashi, Yamaguchi, Hideyo
Format Journal Article
LanguageEnglish
Published Shannon Elsevier B.V 01.05.2005
Elsevier Science
Subjects
Biological and medical sciences
BstF51
CfoI
Dermatomycoses - microbiology
DNA, Fungal - chemistry
DNA, Fungal - genetics
Fundamental and applied biological sciences. Psychology
Humans
Identification
Malassezia
Malassezia - classification
Malassezia - genetics
Malassezia - isolation & purification
Microbiology
Miscellaneous
Mycology
PCR
Polymerase Chain Reaction - methods
Polymorphism, Restriction Fragment Length
RFLP
RNA, Ribosomal - chemistry
RNA, Ribosomal - genetics
Species Specificity
Identification
Malassezia
BstF51
CfoI
PCR
RFLP
Cfol
Yeast
Enzyme
Ribosomal DNA
Method
Standards
Strain
Fungi
Polymerase chain reaction
Specific identification
Fungi Imperfecti
Clinical isolate
Sequencing
Thallophyta
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Summary:A PCR-RFLP method targeted toward 26S rDNA and with 2 restriction enzymes, CfoI and BstF51, was developed to identify 11 Malassezia species. Not only type and standard strains but also 13 clinical isolates were identified successfully in this study. The results of identifications were confirmed by DNA sequencing.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2004.11.016