Unraveling the Consequences of Oxygen Imbalance on Early Embryo Development: Exploring Mitigation Strategies

Although well-established and adopted by commercial laboratories, the in vitro embryo production system still requires refinements to achieve its highest efficiency. Early embryonic development is a dynamic event, demanding suitable conditions to provide a high number of embryos with quality and com...

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Published inAnimals (Basel) Vol. 13; no. 13; p. 2171
Main Authors Marsico, Thamiris Vieira, Silva, Mara Viana, Valente, Roniele Santana, Annes, Kelly, Rissi, Vitor Braga, Glanzner, Werner Giehl, Sudano, Mateus José
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.07.2023
MDPI
Subjects
Animal reproduction
Biological activity
Cell culture
Cell viability
Cryopreservation
DNA damage
Embryo
embryo development
Embryogenesis
Embryonic development
Embryonic growth stage
Embryos
Energy
Energy metabolism
Enzymes
Epigenetic inheritance
Epigenetics
Free radicals
Gene expression
Growth factors
Hypoxia
In vitro fertilization
in vitro production
Lipids
Metabolism
Mitigation
Nitrogen dioxide
Oxidation
Oxidative stress
Oxygen tension
Phosphorylation
Physiological aspects
Physiology
reactive oxygen species
Review
Sheep
embryo development
in vitro production
oxidative stress
reactive oxygen species
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Summary:Although well-established and adopted by commercial laboratories, the in vitro embryo production system still requires refinements to achieve its highest efficiency. Early embryonic development is a dynamic event, demanding suitable conditions to provide a high number of embryos with quality and competence. The first step to obtaining an optimized in vitro environment is to know the embryonic metabolism and energy request throughout the different stages of development. Oxygen plays a crucial role in several key biological processes necessary to sustain and complete embryonic development. Nonetheless, there is still controversy regarding the optimal in vitro atmospheric concentrations during culture. Herein, we discuss the impact of oxygen tension on the viability of in vitro-produced embryos during early development. The importance of oxygen tension is addressed as its roles regarding essential embryonic traits, including embryo production rates, embryonic cell viability, gene expression profile, epigenetic regulation, and post-cryopreservation survival. Finally, we highlight the damage caused by in vitro unbalanced oxygen tensions and strategies to mitigate the harmful effects.
Bibliography:ObjectType-Article-2
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ISSN:2076-2615
2076-2615
DOI:10.3390/ani13132171