Effect of Heterodera schachtii female age on susceptibility to three fungal hyperparasites in the genus Hyalorbilia
Three closely related nematophagous fungi in the genus were compared for their ability to parasitize females and eggs of at different developmental stages. DoUCR50, StM, and ARF were originally isolated from , and , respectively. Phylogenetic analysis and pairwise sequence analysis showed that DoUCR...
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Published in | Journal of nematology Vol. 52; no. 1; pp. 1 - 12 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Sciendo
01.01.2020
Exeley Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Three closely related nematophagous fungi in the genus
were compared for their ability to parasitize females and eggs of
at different developmental stages. DoUCR50, StM, and ARF were originally isolated from
,
and
, respectively. Phylogenetic analysis and pairwise sequence analysis showed that DoUCR50 and StM are more closely related to each other than they are to ARF. DoUCR50 parasitism suppressed 100% of the J2 hatch from 3-week-old
females and 75% of the hatch from 4-week-old females. Eggs within 5-week-old females were resistant to parasitism, and hatch of J2 was unaffected by exposure to DoUCR50. StM and ARF did not reduce the hatch of J2 from
females of any age. Eggs removed from females and spread onto water agar cultures of the fungi were mostly resistant to parasitism. DoUCR50 parasitized only 16% of such eggs from 3-week-old females. Extracellular hydrolytic enzyme production by the three fungal strains grown on PDA or parasitized
females was evaluated using API ZYM (bioMérieux) test strips. All three fungi produced extracellular hydrolytic enzymes when grown on PDA or
females. Trypsin-like protease activity was uniquely detected in DoUCR50 grown on PDA and
females, with the highest activity associated with the fungus grown on parasitized females. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This paper was edited by Patricia Timper. |
ISSN: | 0022-300X 2640-396X 2640-396X |
DOI: | 10.21307/jofnem-2020-093 |