In Vitro Bactericidal Activity of Photo-Irradiated Oxydol Products via Hydroxyl Radical Generation

• Published in: Biocontrol Science Vol. 18; no. 2; pp. 83 - 88
• Main Authors: OYAMADA, AKIRA, IKAI, HIROYO, NAKAMURA, KEISUKE, HAYASHI, EISEI, KANNO, TARO, SASAKI, KEIICHI, NIWANO, YOSHIMI
• Format: Journal Article
• Language: English
• Published: Japan The Society for Antibacterial and Antifungal Agents, Japan 2013; Japan Science and Technology Agency
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bactericidal activity; Hydrogen Peroxide - chemistry; Hydrogen Peroxide - pharmacology; Hydroxyl radical; Hydroxyl Radical - chemistry; Hydroxyl Radical - pharmacology; Lasers; Light; Photolyzed oxydol; Staphylococcus aureus; Staphylococcus aureus - drug effects
• ISSN: 1342-4815; 1884-0205
• DOI: 10.4265/bio.18.83

The oxidative power of hydroxyl radicals has been applied to disinfection systems for the purpose of oral hygiene by utilizing blue light-induced photolysis of hydrogen peroxide (H2O2) in our laboratory. In the present study, the bactericidal potential of blue light-irradiated oxydol products via hydroxyl radical generation was compared with that of 3% (w/v) H2O2. Eleven commercially available oxydol products were used in the present study. Even though a few of the products that contained ethanol, a hydroxyl radical scavenger, as an additive showed slightly lower hydroxyl radical yield as compared with 3% (w/v) H2O2, the blue-light irradiation of each oxydol product for 3 min showed similar or superior bactericidal activity against Staphylococcus aureus to that of 3% (w/v) H2O2. The results strongly suggest that any of the oxydol products tested in the present study can be used as a source of hydroxyl radicals for the disinfection technique developed in our laboratory.